FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6152416
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT441840.7181568996634818No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA212870.3459941590425615No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64500.10483686408721386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGGC19500.022.64590813
CGGCAAT22000.021.4889241
CCTACTA14300.021.4716234
CTTCCGA18950.021.3055821
CGGGCGC20900.021.2929915
CTGTAGG14150.020.48017749
ACCTACT15100.020.10574333
GGACTGT15750.019.93295763
CGCTATG19850.019.81338729
CACCTAC15750.019.27582632
ACACCTA15850.019.15452231
GCAATCG24600.019.0768413
CTAATGC17300.018.54543921
TAGCCCG23900.018.33228934
GACTATC16100.018.21364455
GCGGGCG24450.018.0608614
AGGACTA16750.017.91891753
GTCGCTA22700.017.78145627
GGCGCAT24800.017.66614217
GGCAATC28150.017.5294132
Produced by FastQC (version 0.11.2)