FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5384728
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT469080.8711303523594878No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA155360.2885196801026904No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59370.11025626549753301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTAGG12850.026.04274249
CCTACTA14600.022.92139234
TATGCCG6250.020.97556146
CGGCAAT19700.020.8454721
ACCTACT16450.020.76306333
CTAATGC17050.020.63848121
GGCGGGC17100.020.57870913
GTTACCT17600.020.19029414
ACACCTA17450.019.7707331
CGGGCGC17400.019.62853815
GGACTGT15650.019.61921563
CGTATGC6600.019.34050644
CTTCCGA19100.019.3322071
CACCTAC17700.019.29656632
GACTATC16450.019.2946155
TACTCTA18900.019.1684044
CTATCTA18350.018.42519838
TCTGTAG19400.018.31696948
GCAATCG21550.018.2522343
GTATGCC7200.018.20778545
Produced by FastQC (version 0.11.2)