FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5559654
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT623661.121760454877228No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA211440.3803114366469568No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG58140.10457485303941576No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTAGG16350.022.9993949
CCTACTA16300.022.85911234
TACTCTA18450.021.5056214
CACCTAC18200.021.04140732
GGACTGT16900.021.02625863
ACACCTA18150.020.909131
ACCTACT18200.020.4727233
CGGCAAT21800.020.4182681
GGCGGGC19700.020.1394213
GTACTCT19800.020.0391483
CGCTATG19300.019.84197629
CTAATGC19650.019.66448421
CGGGCGC20300.019.54328715
GACTATC18550.019.34197855
CGCGGGG1258.7384215E-519.3229621
GTTACCT20150.019.17566914
AGGACTA18800.019.08494253
GAGGCGT21750.018.71708153
TAGCCCG22350.018.21493734
TGGACAC20900.018.15790228
Produced by FastQC (version 0.11.2)