FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2085341
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72820.3491994834417968No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT23740.11384229245960252No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23190.11120483412545No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGC22120.10607377882082594No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGTT8000.025.442757
ACCTACT7200.025.39605333
CCTACTA6950.025.3161634
GTACTCT7950.025.1724453
TACTCTA8100.025.132264
CTGTAGG6600.024.56721749
GTTACCT7750.023.59318514
CACCTAC7500.023.000232
GGACTGT7050.022.51137463
CTAATGC8150.022.434721
ACACCTA7700.022.40279231
GACTATC7250.022.3646455
CGTATGC2057.4578566E-1121.8771944
AGGACTG7750.021.81309162
CTATCTA8400.021.76752338
TATGCCG2101.0186341E-1021.35630246
TCAGGAC7700.021.05862460
TCTGTAG8300.020.78231848
ACCCGCT7700.020.6100739
AGGACTA7600.020.4268353
Produced by FastQC (version 0.11.2)