FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7080392
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273520.3863062949057058No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT96340.13606591273477514No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA91510.12924425653268914No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT86980.12284630568477No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGC81750.11545970901046157No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72800.10281916594448444No Hit
GCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGG71950.10161866744101175No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG2300.025.5045851
CCTACTA16450.024.32851634
CTGTAGG17050.024.07891749
CTTCCGA18800.023.860671
ACCTACT17950.022.87242333
TATGCCG5650.022.59283346
GGACTGT17900.021.77893663
CACCTAC19150.021.07839632
AGGACTA19050.021.00734953
GACTATC19150.020.71734855
CGTATGC6400.020.4841544
CGCTATG35050.020.1783829
TACTCTA22450.020.1328894
ACACCTA20400.020.12520631
GTACTCT21300.020.085993
CTAATGC21050.019.9947521
GTTACCT21000.019.71476614
AGGACTG21200.019.03975162
AACGTCG37700.018.94262924
TTCCGAT23750.018.8860032
Produced by FastQC (version 0.11.2)