FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6505742
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89380.13738632733975617No Hit
TTGTACTCTAGTTGTTACCTCTAATGCTGGACACCTACTATCTAACCTCTGTAGGACTATCAGGACTGTTTGATC87680.13477325107574203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGGG2200.025.09641
CTTCCGA22450.022.7488521
TACTCTA30800.022.5164224
CCTACTA27600.022.3750834
CTGTAGG26850.021.84331749
GTACTCT32600.021.1678353
ACCTACT30250.020.18669333
CTAATGC32500.019.9563821
TCTAGTT34850.019.8977477
CACCTAC31000.019.80959532
ACACCTA31150.019.71390231
TATGCCG8450.019.59679846
GACTATC30650.019.24773455
CGTATGC8250.019.23584244
CGCTATG18400.019.12418629
GGACTGT30550.018.85931663
GTTACCT35150.018.35426114
TAGTTGT37400.018.2649129
CTATCTA33450.018.2555338
TTCCGAT28650.017.704222
Produced by FastQC (version 0.11.2)