FastQCFastQC Report
Sat 7 Oct 2017
HKVL7BGX3_n01_ap-tp_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVL7BGX3_n01_ap-tp_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10906025
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTTTTACCCTTTTGTTCCACACGAGATT179900.164954692475031No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT172800.15844452951464902No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACTTAGAGGCGTTCAGTCATAATCCAGC167390.15348396872371006No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCTATGATGTTATCC149450.13703434569423784No Hit
CTGACATGTGTGCGAGTCAACGGGTGAGTAAACCCGTAAGGCGCAAGGAAGCTGATTGGCGGGATCCCTCGCGGG143040.1311568605426817No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131260.12035549157461127No Hit
CTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGGCGAGCGAACCGGG126500.1159909316180735No Hit
GCGGGATTACCCGCTGAGTTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCTTAGTAACGG125960.11549579246334023No Hit
GCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTACGTGGAGGCATCCCTTCAC112430.10308980586418975No Hit
GCCGGGTCGTGCCTCCGGCGCTGTTACTTTGAAGAAATTAGAGTGCTCAAAGCAAGCCTACGCTCTGGATACATT109270.10019232488463944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA26700.029.080361
CGCGGGG2650.028.6487261
CCGGCAT8950.027.7611831
GCCAGTA33500.025.2377011
TTCCGAT31600.024.7867432
CGGGATT45850.023.4798852
CCGATCT33450.023.0027354
CTGCCAG19200.022.1046982
ACATCCG34150.021.51740855
CGCTATG68550.021.48975429
GAGGCGT62350.021.19187453
AGGCGTT63000.021.0823654
ACCCGCT48000.021.0596589
AACGTCG69800.021.05596524
CAGTAGT40600.020.991223
CCAGTAG41000.020.8712542
GGGATTA50950.020.790313
GTCGCTA73200.020.26620127
CAACGTC72700.020.26340523
GGCTGCC71800.020.22873343
Produced by FastQC (version 0.11.2)