FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47425316
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATC151950.016.66712869
GAGCGAT204850.015.34432469
ACGAGCG328350.015.2999469
CGAGCGA276800.014.34744669
TAGAAGA346500.013.9998731
TCGAAGA129750.013.8273191
GCGATCT100250.013.42287769
TCGACGA50400.013.2804631
TAGGAGA200500.012.733841
TCGGGAA151250.012.7057951
TGCCGAC902400.012.46093669
GCCGACG739700.012.14648869
TCTTCGA142200.012.1314411
TCGGAAA125950.012.1078251
TCTTTGA360950.011.9865031
TATTCGA40650.011.9674221
TCTTGAA233850.011.8620761
CGACGAG513950.011.83577769
GACGAGC473800.011.48423469
TCGGAGA151200.011.4093311
Produced by FastQC (version 0.11.5)