FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr99.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr99.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8865510
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATC64000.022.15721369
GAGCGAT89900.020.45600369
GCGATCT42100.020.40659169
ACGAGCG181400.019.1343669
CGAGCGA136800.018.91596269
CTATACT22250.017.8311544
CTACACT36950.017.7398834
TATACTG22000.017.24985
TAGACTG37650.017.135265
GACGAGC244150.016.9298669
GTATTAG15200.016.7969951
CCTATAC21700.016.6932623
CGACGAG312850.016.1236969
GTCCTAC25150.015.9134181
CTAGACT33700.015.458194
TACACTG45950.015.316475
TACACCG9850.015.06073955
CCTACAC45650.015.039173
ACACCGT12050.014.88779456
GCCGACG508300.014.88578669
Produced by FastQC (version 0.11.5)