FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr98.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr98.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515589
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATG400.002593437434.5042756
AGATTCG400.002595906634.4975785
TGTACGG605.4203335E-428.747985
CCTTATG500.00773001927.6007372
GACGTGT500.00773001927.60073753
CGTCTAG756.1558996E-527.6007371
CATCGTT500.00773001927.6007372
GCATCGT500.00773001927.6007371
CCGATCG500.007733679427.5980641
TAGGACG1457.021299E-1026.1705744
CTAATCG700.001333395724.64112535
TCGTGTG1001.5623402E-524.1483029
GTTGTAC1301.8428E-723.8852541
GTGTTAA750.001991230523.0006141
CGAGAGG1052.2788607E-522.99838414
ACTCCGG902.1251554E-422.9983848
TTAGTCG750.001992354422.99838339
AGTCGGC750.001992354422.99838341
GAGCGAT6400.022.10858769
CAGTACA2207.2759576E-1221.9530014
Produced by FastQC (version 0.11.5)