FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr97.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr97.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677151
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14150.2089637318707349No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATAA2700.026.83316219
GTATAAG2450.026.75492920
GTACTAC951.0499889E-525.4208891
CGCCTCA2450.023.938628
CGCTCGA902.1251928E-422.99985310
TTAGTAC1352.65607E-722.9998533
CTAGCAC1502.8827344E-822.9998533
CTCAGAT3300.021.95440511
GTGTATA3050.021.49166518
TAACCTC1455.286656E-721.4136565
CTACGCT1304.9171613E-621.2306334
GTCTCCG1507.3234514E-720.6998673
TAGGACT1851.1743396E-820.5133824
TATAAGA3900.020.34602421
GTAGGAC1901.5957994E-819.9735553
TCTCCGC2655.456968E-1219.5281754
TACACTG3200.019.4061245
TACTGTG2153.1413947E-919.255697
CAGTACT2153.1413947E-919.255694
ACTATAC1451.2636179E-519.034363
Produced by FastQC (version 0.11.5)