FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3835469
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG322780.8415659206214416No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTCA15050.025.2157048
GTATAAG13600.021.56257220
GTCCTAC14450.020.0556931
GTAGGAC14750.018.945583
TGTATAA18800.018.7181519
GACGAGC29950.018.43126169
ATAAGAG17750.017.88128522
TCTCCGC15900.017.7922824
CGACGAG36650.017.79179669
TATAAGA20150.017.63529621
GCCGACG72400.016.96458869
CTATACT10800.016.9303934
TCCGCCT18700.016.7886096
GTCTCCG11100.016.783623
TAGGACG10900.016.4585574
GTGTATA17950.016.33710318
ACGAGCG20450.016.02736769
TAGACCG3251.2369128E-1015.9229235
CTCAGAT22600.015.87595311
TATACTG11600.015.7627785
Produced by FastQC (version 0.11.5)