FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5504724
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG162370.29496483384089733No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTCA22350.024.6968068
GTATAAG19100.021.13288120
GTCCTAC21600.020.6037431
TGTATAA22500.019.62608519
ACGAGCG57850.019.08570169
TATAAGA26750.018.31347521
GACGAGC81900.017.69403569
ATAAGAG24800.017.11022622
CGACGAG102250.017.07452869
GAGCGAT28800.016.89227369
TACACTG31550.016.7299395
CGAGCGA43050.016.67061669
TAGGACG17600.016.6609844
GTAGGAC21950.016.3453123
GTATTAG11100.016.1618291
TAGACTG19950.016.0820855
CTACAGT30750.016.042994
CTATACT15600.015.9221714
GTATAGG14150.015.847731
TCCTACA31100.015.6411592
Produced by FastQC (version 0.11.5)