FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2721235
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41810.1536434743783613No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC9850.024.1679251
TATACCG2350.023.489335
CGCCTCA8850.023.3893688
AGCGATC15500.020.47927569
TAGGACG6450.020.325184
ACGAGCG41500.019.78733469
TACACTG14200.019.4365965
CTACACT11300.019.2341354
GAGCGAT21400.018.54139969
GTATAGG6700.018.0226961
CTATACT7500.017.4796564
GTAGGAC10950.017.3284263
CGAGCGA30650.017.11084769
GTATAAG8150.016.93187120
GTATAGA6700.016.4778941
GACGAGC56400.016.15039869
TAGACCG2351.8199717E-716.1489145
TCTAGAC8850.015.9827363
TTAGGAC10150.015.9750553
ACCGTGC5650.015.8757938
Produced by FastQC (version 0.11.5)