FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9537545
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG583640.6119394456330219No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG25300.021.81812920
AGCGATC46550.021.0498369
ACGAGCG151150.020.65809869
GAGCGAT68900.020.2808369
TATAAGA32650.018.8085321
TGTATAA31600.018.66925819
GACGAGC210400.018.26793169
CGCCTCA40450.018.2515458
TATACTG24850.017.631475
CTATACT22400.017.5577164
GTCCTAC26950.017.5390031
CTACACT37550.016.9970284
CGAGCGA111400.016.7866569
GCCGACG484600.016.11917969
GTCCTAG36700.016.0758191
CGACGAG264200.016.03671869
ATAAGAG36800.015.93695922
CTAGACT32250.015.8322714
TAGACTG36300.015.7765725
GCGATCT30950.015.71840169
Produced by FastQC (version 0.11.5)