FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2016167
Sequences flagged as poor quality0
Sequence length75
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG91510.4538810525120191No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG953.068877E-421.7872184
GCCGACG80150.020.4049369
GCACCGT3750.020.2389096
GTCCTAC7700.019.266151
TAAGTCG900.005737672619.1656345
CGTCTAG2354.6020432E-1019.0850241
TGCCGAC105100.018.35140869
CTACACT8700.017.8429814
ACGAGCG22200.017.56247769
GAGCGAT10900.017.40990869
TAGACTG6750.017.376845
CTAGGAC9500.017.0666543
CGACGAG38400.016.98207969
CCGACGT5150.016.74916369
CCGACGA50750.016.7247469
GTTATGA3106.002665E-1116.6934761
TCCTACA8900.016.6684682
GTAGGAC9200.016.4982933
TCTAGAC6350.016.2975253
TCGATCA1503.5275597E-416.09953127
Produced by FastQC (version 0.11.5)