FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1878816
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105620.5621625534379098No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGAC8350.019.41893
GTCCTAA8300.017.4585251
ACGAGCG11700.017.39812769
TATACCG3800.017.2497675
GCCGACG41150.017.18780369
GTTCTAA4450.017.0568561
TAGGACG8650.016.7512194
TCTATAC4950.016.7270473
CTACAGT11900.016.5249864
GTCTAGA3006.8030204E-1016.1006411
CGACGAG19800.016.03094169
TGTAGGA10150.015.9760042
GTCCTAC9150.015.8366951
TTAGGAC7950.015.622433
TGCCGAC51750.015.6006269
CTATACA5350.015.4764274
CCGACGA27300.015.41819569
CGCCTCA4101.8189894E-1215.1461378
TAAGACT5300.014.9714964
TCCGTGA6250.014.90300626
Produced by FastQC (version 0.11.5)