FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1876469
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72090.3841790085527659No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC13350.021.9670981
GCCGACG36450.021.86569
TAGGACG16300.020.9530814
GTAGGAC14700.020.18283
AGGACGT17400.019.8272575
ACGAGCG9350.019.55686869
CTTGACG8400.019.30376459
CGTGATT8250.019.23604428
CCGTGAT8300.019.12016727
GGACGTG18200.018.7661726
GTCTACG1301.1811997E-418.5776041
TTCTCGC8400.018.48183642
GACGACT8500.017.85852662
TTGACGA9100.017.81886160
TCCGTGA8950.017.73107726
CGAATGT2551.36788E-917.58794233
GACGTGA11000.017.5633437
CCGACGA21800.017.40889569
TGCCGAC48500.017.35733669
ACGACTT8350.017.35300463
Produced by FastQC (version 0.11.5)