FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1727204
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19220.11127811190803172No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAC18500.028.7199671
TCCTACA22550.022.3378452
CTACAGT25200.022.3149994
TACAGTG25900.022.2446985
GTAGGAC19250.022.0436843
CCTACAG22750.021.8368953
TCTCGCC9800.021.12260843
CCGTGAT9850.020.66453227
TCCGTGA10200.020.6319126
TCCTCGC13150.020.46403344
TTTCCGT10950.019.8488924
CCTCGCC14000.019.71443445
TTCCGTG10350.019.66624525
CGTGATT10000.019.6645828
CGACGAG25400.019.424569
TTCTCGC10550.019.29398542
TGTAGGA22950.019.2425332
AGTGGAC17650.019.15548
TAGGACG23900.019.0539864
TTCCTCG14550.018.96921543
Produced by FastQC (version 0.11.5)