FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316977
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAATT150.002353968869.0000143
GCGTCCG350.001346342839.42857417
TTTCCGT1353.0377123E-1028.11110924
GCCCCGA500.007726609227.59999830
CTACTCG500.007726609227.59999813
CGACGAT500.007726609227.59999868
GCCGCAT658.6432067E-426.53846252
TTCCGTG1208.4774E-825.87500225
CCGGTTG700.001331717624.64285941
TGGTGCG700.001331717624.6428599
TACACCC851.4400831E-424.3529425
ACTACAC1001.5590258E-524.1499983
GTATAGC1001.5590258E-524.1499981
GTCCTAC2600.023.8846151
TCCGTGA1301.8386163E-723.88461526
ATGGCGA2650.023.43396217
ATAAGGG750.001989857823.03
TCACGGA1052.2740425E-522.99999836
GGCGAGA1352.646666E-722.99999819
CTACACT1554.0628947E-822.2580664
Produced by FastQC (version 0.11.5)