FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7245188
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG456030.6294246608921673No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTCA75250.050.0171938
TCTCCGC66800.048.0301064
GTCTCCG42150.046.162423
CGTCTCC41050.043.957352
CCGCCTC85450.043.2796557
CTCCGCC86950.042.89075
CCGTCTC40000.042.6961021
TCCGCCT78900.042.3258256
GTATAAG71650.040.54319420
CTCAGAT90400.038.20055811
ATAAGAG79900.037.3482622
TGTATAA85500.037.0423619
TATAAGA90050.036.3198221
CCTCAGA110950.035.29170210
AAGAGAC96750.034.65878724
TAAGAGA86350.034.51854723
TCAGATG91000.033.9301412
GCCTCAG105750.032.78619
GATGTGT82900.032.66932715
AGATGTG89050.032.65883614
Produced by FastQC (version 0.11.5)