FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3918713
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG180820.46142700422307015No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTCA14200.028.4250628
GTATAAG14400.023.47954620
TCTCCGC14800.022.6109854
CGTCTAG2750.022.5827581
CTCAGAT18400.022.12450811
TGTATAA17850.021.64740619
GTCCTAC17300.020.9401781
TATAAGA19300.020.73608621
AAGAGAC20800.020.5665924
ATAAGAG18200.020.09270522
GTAGGAC16550.019.5947323
CCGCCTC20250.019.4215437
GTCTCCG9300.019.2898943
CCTCAGA22100.019.2009310
CGTCTCC10400.018.5776962
TAAGAGA20100.018.19339623
TAGGACG17150.017.9033934
GACGAGC37050.017.78593869
ACGAGCG25350.017.6928269
CTAGGAC9950.017.6830233
Produced by FastQC (version 0.11.5)