FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr85.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr85.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2746088
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATC25850.024.69221769
CGCCTCA8150.024.551158
CCGTGAT8600.023.26649527
GTCCTAC17750.022.5476151
CGTGATT8600.022.46420328
CGATCTA9050.022.11201769
TCCGTGA8850.021.82962226
TAGGACG18450.020.7560084
GCGATCT17400.020.62210569
GTAGGAC18550.020.2721523
GAGCGAT36600.019.98497869
TACACGG3000.019.5499175
TAGACGC2951.8189894E-1218.7117864
CTACACG3150.018.6189694
CTAGACG2053.828609E-818.5121173
TTGACGA8950.018.50305460
TTCCGTG10000.018.28425625
AGGACGT19600.018.1300265
TTTCCGT10300.018.08664324
TACACTG15400.017.9225
Produced by FastQC (version 0.11.5)