FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences398554
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCTA150.002354890668.9964936
GACGTCC200.007353357551.75385768
CGTCTAG350.001347096839.4265671
TGTCACG500.00773087627.59859748
GCCCAAT500.00773087627.5985971
CGATACA500.00773087627.59859714
ACTAAGC658.649705E-426.5371123
TATACCG700.001332714524.6416035
CGACTTG851.4402623E-424.35475764
TAGACTG1452.0076186E-823.7918915
TTAGCAC750.001991341422.9988293
CCAGGAC1653.1304808E-922.9988293
AGTACTC1052.2768414E-522.9988278
GGGTTGA953.0618155E-421.7883641
GTAAGCT953.0618155E-421.7883644
CCTATCC953.0618155E-421.7883643
CTATACA800.00289700221.5614014
GAACCGT800.00289700221.5614016
TGTACTG1605.6978024E-821.5614015
TACGTGT800.00289700221.56140112
Produced by FastQC (version 0.11.5)