FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1701769
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTCA6750.036.2877168
CCGCCTC8450.028.5789577
CCTCAGA8600.027.67933710
CTCAGAT8400.026.69556411
GCCTCAG7500.026.2191529
TCTCCGC8350.025.6166924
CTCCGCC10450.025.4209775
GTATAAG6950.024.81934220
TCCGCCT9250.024.2431726
ATGTGTA6700.024.2007116
TGTATAA9050.024.01579719
AAGAGAC10200.022.99857924
TATAAGA9200.022.87425821
GTCTCCG4000.022.4249343
CTAGACG800.002900048421.5624353
GTCCTAC4200.021.3577081
TAAGAGA9200.020.9993223
CGTCTCC5150.020.767542
GTGTATA8000.020.6993318
ATAAGAG9600.020.48371322
Produced by FastQC (version 0.11.5)