FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1691233
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATC18350.024.06812769
GCGATCT11950.023.96506369
GTATAGG4000.023.2887671
CGCCTCA5350.021.9244828
CGTACGT800.002900070521.56239910
TAAGACG1551.0053718E-620.0315724
GTATTAG2800.019.7153591
CTACACT6850.019.6416634
TACACTG9950.019.416425
CGATCTA6250.018.77013269
GTCTAAG4250.018.6716041
CTATACT4450.018.6061024
CTAGACT5700.018.1572724
TAGACTG5000.017.9393845
GAGCGAT25250.017.90104369
GTCCTAA6000.017.8259721
TACACGG1753.2106564E-617.7422495
GTATAGA3950.017.4693051
TAGACAG6950.017.3735055
GTCCTAG6400.017.2509381
Produced by FastQC (version 0.11.5)