FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515430
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCCG1201.8189894E-1234.499373
CTACACG652.3193446E-531.8455724
CGTCTAG450.004620763530.6661071
GTCCTAC2900.027.3615681
TACGTAT809.5529904E-525.8745257
TCTCCGC1759.094947E-1225.6281054
TACAGTG3950.025.3286515
TAGGACA2050.025.243444
ACGTACT700.001332990824.64240629
TAGGTAG1001.5617103E-524.1495595
CTACAGT3000.024.1495574
CCATGCG1151.6948889E-623.999569
TAGACTG1902.5465852E-1123.6048325
CCTACAG3400.023.3378093
CGCCTCA2103.6379788E-1222.999588
TTAGTAC750.00199175222.9995783
TCGGTAG750.00199175222.99957846
CGTCTCC1854.820322E-1022.3779722
CCGTCTC1854.820322E-1022.3779721
CCCTAAG1704.425601E-922.3231221
Produced by FastQC (version 0.11.5)