FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816106
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTCA7800.035.3819738
GTCTCCG4700.034.4964753
CCTCAGA8650.032.3057310
CGTCTCC5700.029.0512642
TCTCCGC8700.028.9453164
ATAAGAG8350.028.50763922
TATAAGA9450.028.47641821
CCGCCTC9650.028.2414177
GTATAAG8050.027.85736720
GCCTCAG8300.027.8488529
TAAGAGA8700.027.36153223
CTCAGAT8450.026.53794111
CTCCGCC10900.026.2686825
AAGAGAC10300.026.12426624
CCGTCTC6550.025.8079451
TGTATAA10050.025.40318919
TCCGCCT10200.024.0129136
GTGTATA9450.023.73034918
AGAGACA12950.021.84393125
GTTCTAG1154.6061934E-520.999011
Produced by FastQC (version 0.11.5)