FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1435513
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG1800.026.8318963
TAGACGC3050.024.8839154
CGTCTAG1803.43789E-1022.9979671
GTATTAG4750.021.0612961
TAGACGT1206.3911255E-520.1239244
TTAGAGC2402.7284841E-1120.1239243
GTATAGG4150.019.9500451
TTAGGAC4500.019.9322663
ACCTACG2603.6379788E-1219.90347511
TAGGACG4400.019.6012234
TACGTAG1258.753571E-519.31694826
CTACAGT7150.019.2996654
TATACTG3800.019.0654345
AGCGATC11950.019.05602869
GTCTACG1852.6466296E-718.6470011
CGCCTCA4650.018.548048
CCTACGG2801.2732926E-1118.48179812
GTCCTAC5800.018.4380251
TAGTCCG3001.8189894E-1218.39965630
TAGACTG4700.018.3507215
Produced by FastQC (version 0.11.5)