FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2165242
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG854.429612E-628.4125161
CGCCTCA8550.025.017058
CCTCAGA10850.023.21152310
CCGCCTC9600.021.9214427
CTAGACT4000.021.5620734
ATAAGAG10850.021.30323622
TAGGGGT4250.021.1054654
TCCGCCT9000.020.6995936
GTATAGG4050.020.4449841
GTATAAG9300.020.40329420
GTGTAAG5950.020.2946531
TATAAGA11950.019.92056821
TGTATAA12200.019.79514919
GCCTCAG9950.019.7634289
AAGAGAC12600.019.71344224
TAAGAGA11350.019.4529123
CTCAGAT10550.019.29345911
CGATCTA7700.019.26763269
CTCCGCC12400.019.1972035
ATGTGTA10150.019.03454816
Produced by FastQC (version 0.11.5)