FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6809783
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATC73750.027.55652469
GCGATCT45500.023.20497969
GAGCGAT105100.020.9782569
CGATCTA24200.018.82043669
CTATACT15800.018.3410074
CTACACT29050.018.050884
CCTACAC34150.017.7796383
TACACTG36350.017.2731345
TACACCG9200.017.2494075
TAGACTG28050.017.2186585
GTATAGG15200.016.7964611
GTATAGC14800.016.7841931
TAGGACG14100.016.6376064
GTCCTAC20500.016.4930861
TAGGTCG7950.016.495
CCTATAC15900.015.8389633
CGAGCGA143050.015.75057768
ACGAGCG179450.015.709043569
CTAGACT25350.015.6502354
TATACTG16600.015.378995
Produced by FastQC (version 0.11.5)