FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1282394
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG2150.030.4894731
CTAGACT4700.024.2233354
TCTAGAC5400.023.6388233
GTATAGG3950.022.7096821
TAGACTG5800.022.6033845
ACGGCTA2600.022.55762934
GTCCTAC4500.022.2341371
CGCCTCA3600.021.0832758
CGGCTAT2800.020.94637135
CTAGACG1507.3337105E-720.6999423
TTGACGG3000.020.69994231
TACACCG2850.020.578895
CCTATAC3800.019.9736273
CGTACGG1751.4731086E-719.7142337
CTACAGT6850.019.642284
CTACACC6500.019.6384074
ACGTACG1601.3621066E-619.40619736
TAGACGC2002.8812792E-818.9749454
TCTACAC4850.018.4947933
TAGACCG1702.4327837E-618.2646545
Produced by FastQC (version 0.11.5)