FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2016760
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTCA8000.032.7746248
CTCAGAT9800.027.4588711
CCTCAGA11950.025.69426310
CCGCCTC10750.023.7485647
CTCCGCC11400.023.6055765
TATAAGA10350.023.33306521
TCTCCGC9100.023.1266824
GTATAAG9050.022.49145520
GCCTCAG10800.022.3608559
TGTATAA10500.022.342619
GTCCTAC5900.022.2217351
TAAGAGA10250.022.21437823
TCCGCCT10350.021.3336166
ATGTGTA9800.021.12220816
AAGAGAC11700.020.93565824
GTGTATA10300.020.76675218
ATAAGAG10700.020.63527922
GTCTTAG3150.019.7155251
AGATGTG10550.019.62062814
GTCCAAG2303.45608E-1019.5012261
Produced by FastQC (version 0.11.5)