FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATGTCTTGTTGTCTGTGTGTCCTGTTTCTGTGTATAACTCCTAATTAGGAAGTGCTCTACCACCTGTCTCT11.1494252873563218No Hit
GTGTGCTTCAGCACAGCTGCACCTAGGGGACTGAAATGACATTGCAGAGACCCTTTGTGCTGCTTTTTTGGCCCG11.1494252873563218No Hit
CTATTCAGCAAGCAGGCACTGACTCTAACTTAACTTTCAGTGACAACTCTTCTCCCTATTTGCTGGTGTTGTTCA11.1494252873563218No Hit
GTCCAGGTCCTACAGTGTGCATTTTCATTTTTCACGTTTTTAGTGATTTCTTCATTTTTCAAGTCGTCAAGTGGA11.1494252873563218No Hit
ATATTCTGGCATCGTGCGAGCAAAGTCTTCAGTGCCAATCTGGCGGGCCAGGTTGATGATGTGCTCTTTGTCGTA11.1494252873563218No Hit
GTACAGAGGAGGAGAAACGATTGAAGGGAGGAAGGAAGGAAAGTGCCACTAAGAAGTGAGCTAAGAGGTAAAGGA11.1494252873563218No Hit
CTATAAAAACTTCGCAACTTTGGGCAAGCTTAGAAGATTCCGTCTCCAAACAGAGTTTGACACCTCACAGAACTC11.1494252873563218No Hit
GAAGAATGTCATATAACACAGAAATGTCCTTAAAACATTAGTTTAATGTGTATAATTTAATAATAATGTTATATA11.1494252873563218No Hit
CCTATGGCGTGGGTAATGCGCCACAAAACAAAGCCTTCCTGCAGGAATTACAAGAAGCCAGCGATCGCGGTATTG11.1494252873563218No Hit
GTATGGCACTGGGCAGAAGCCATGTGTGAAGGCCGCTTGGAGGGAGAACGGGAGACTTGGGCCTGGCAGGCATCT11.1494252873563218No Hit
GCGTCAGGTGGGTATGGCGACTCTGGCGGTGCCAATCCACAATGCGGAAGAAGCGTTTAACCCGAATGCGGTGAA11.1494252873563218No Hit
GGCATTTGTGTGACGAAGTGAGTCATCTTCCGGTCAGCTGTTGGTTCAGGCACAGGATCATTAGAAGGGTGGAGA11.1494252873563218No Hit
CACCCATGGTGAAGATATACCACAAACCAAAAGGAGCAGGCCGGAGGAATGACAATGGGGCTGGGGGGCCGTGTT11.1494252873563218No Hit
GCATTATGTTGACAATCAAGTATGTTCCTTGTGTTTCTGTTGGTAAGCTGTCTCTTATACACATCTGACGCTGCC11.1494252873563218No Hit
GATTAAAGGCATGTGCCACTACTGCCTGGCTTGTACCACTGCATATTATTTCAGTGAATTACATACCTGTCTCTT11.1494252873563218No Hit
CCCCTGCGGCGCTTTGCCCGCCTTCGGTTAGCCAGTAATTTGGCAACATGGCCGACCAGTATGGCCCCCAGACGC11.1494252873563218No Hit
GTCTAGACGCACCTACGGTGAAGAATCATTAGTCCGCAAAACCCAATCACCTAAGGCTAATTATTCATGCTTGTT11.1494252873563218No Hit
GATTAAGGTTGCTTCAAATAAAATATAAAATATAATTAGTTCAGTTGCTGAAAAGGTTATGATTAGGAGAATTTG11.1494252873563218No Hit
CTAAAGCAGTGAACCTGTTGTTGGGAGGTGTGTAGATATTGTGTTGTTGTGAGGTGTGTAGATATTGTGTTGTTG11.1494252873563218No Hit
CGCCACCACCCACCGGGATTTCAAAGTCGAAGTTTTCATAGCCAGAATAAGGACTGTCTCTGATACACATCTGAC11.1494252873563218No Hit
CCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTCTGTCTCTTATACACATCTGACGC11.1494252873563218No Hit
GGCTCATAGACATTACGGTGCCAGTGAAATTCATCACCGCCCTCTTCGGCGGCATCTGCTGCGCGTTTGAGCTCA11.1494252873563218No Hit
GTGGGGGGGGGGGTGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGCGGTGGGGGGGGGGTGGCGGGGGGGGGGGGG11.1494252873563218No Hit
GTGCCAGCTGCCAGCTGCCGGCTGGGGGCGGGATGCGCTGCCCTGGGGTCGCTGGCACAGCTGTGGTGGGGCCCC11.1494252873563218No Hit
AGTCCTGCTAAAGCAGGTGAGGTGAAAGTGCATCAGTTCCTTCCCCGTGAGCATGTTCTGCTCAGGTGCTCTTCA11.1494252873563218No Hit
GATCCAGCAACGTTGCCACGCCAAACGGCGCGCGGCGGGTGATCCAGTGTAAATTAGTCGAGCAATACGCCATTA11.1494252873563218No Hit
AAATGGACCCGAAAGTGGTGTTTGTTACCACCTGTTTGATTGCCGGTATCGGCAGTATTTCGATGCTGTCTCTTA11.1494252873563218No Hit
ATCCGCTTCTTCCAGCAGGATCTGCCGCACTTCCTGTTCCGAGGAGGCGATCGCATCTGCCTCGCTCAACGCTTT11.1494252873563218No Hit
CCTTTATTCTAGTCACAATTCTATCTCTAGGCCTAGCATATGAATGAACACAAATAGGATTAGAATGAACAGAGT11.1494252873563218No Hit
AAGCTACACGTTTCATGCCCTATGCGTCACATGAAGCATGTGACACCTGTCTCTTATACACATCTGACGCTGCCG11.1494252873563218No Hit
TCGCAGGAGTGGCGAAAATGCATGAGTTGTCTCTTTGCCAGAGCGCCGTTGAAATTATCCAACGGCAGGCGGAGC11.1494252873563218No Hit
TGCCTGCTCCCCGCCCCCTCGGCCTGTCCGGGGAGGGGAAGGGCTGTCTCTTATACACATCTGACGCTGCCGACG11.1494252873563218No Hit
GGCTGGGCTAGTACTGGTCCATGCTTGTTAGCAGATGCTGCAGGTGTAATTAACTCTGCTACAGGCTATGCCCTA11.1494252873563218No Hit
CACACACACAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGATTAGTGTTACATGTGAGTGATACTACTTTTTA11.1494252873563218No Hit
ATACTGCGATGTGATGGGGCGTGTCGACATCATCACCGGCACGCTCGGTAAAGCGCTGGGCGGGGCTTCTGGTGG11.1494252873563218No Hit
CATGTGAAGTGGAAACGACTAGTTTCTTTGGAACGTTATTTCAAATTATGATTAGCTCGTGCACACAGGTGAAAG11.1494252873563218No Hit
CCCCTGCCCTGCACAGTGGAAGGAGTGTGTCCTTAGCTAGCTGATAATAAAACCTCTTGCAGTTTGCTTCAGGTG11.1494252873563218No Hit
CCATATGACCATCAAAGGGCAAAAACTGGTGCTGCCTTCATGGAAATTCGCCCTCGCTCAGATGCTGATTTCCAG11.1494252873563218No Hit
GGATGCCCATGCTCTCTGTTTTTTGTAATCCACAAATTGACAGGCAAGCAGGGCATATAAACAATGAAGGCAGCT11.1494252873563218No Hit
CTTTTATACTCGCCTAATTTATTCCACTTCACTAACAATATTTCCAACCAACAATAACTCAAAAATAATAACTCA11.1494252873563218No Hit
TCTCTAGGCTGCCCGGGCCGCGCAAGCCAGGACTAGGAGATTCCGGTGTGGCCTGAAAGCCTGGCCTGCTCCGCG11.1494252873563218No Hit
CTCCAAGCAGCCATGACGCTCGTCTGCAGCTGGGCGCACCGCCCCGCGGGATGCTGGGGGCGGAGCCACGTGGCG11.1494252873563218No Hit
ATCGTATTCTGATCAAACTGGCTTCTACCTGGCATGTTATCCGTGCTGCAGAACAGATGGAAAAAGAAGGCATCA11.1494252873563218No Hit
ATTCAGTATGCCGCGCATCTAATTTATCCCATAGAAGCCAATCCGTTATGGTCGCAAGAATGGATAGGCTGGGGG11.1494252873563218No Hit
CTACAAGCTTGCCCACGGGCAATCTGATGAAGGTGTTTTGTCCATCTCAGTTCCCTCTTCCAAGGTTACTCTGGC11.1494252873563218No Hit
GCACGCATTTGCTCCAGGCACTTGGTTACCCTGATGTACCAGCTTCCCATCGACATGATCACCAGCAGCATCAGC11.1494252873563218No Hit
GCTGTGAGCTGGTGATGAGGAGATAGTGGGGTTGCCCTGGTTCTGTGAGCTAAAAAAAAAAAAAAAAAAAAAAAA11.1494252873563218No Hit
GTCCAGCACAACGCTGGCTCAGCTAGCGAAAGCCGGCGGGATAGCCCATGCCGGTGACATGCCCCAAAGGAGGGG11.1494252873563218No Hit
GAACTGGATATGAATGGGTATCCTCAGACAATAAGCAAACTACTGACGGCTGCAGGCCTGTCTCTTATACACATC11.1494252873563218No Hit
GTTCTGGCTTTCACCGCAGGTACTTCCGTAGCTGCGACTTCTACTGTAACTGGCGGTTACGCACAGAGCGACGCT11.1494252873563218No Hit
GTGTTGATCTGGAAGGGTTGGCGCGTCATCGCGGTTCGGCGTTTGGTCGCACGTTACAACCACAACTTAGCCAGG11.1494252873563218No Hit
GTCTAGAATAATGGAGATGCGAATTATTCCGTAACTACCTAATTTTAGAAGAATAGCTGCTAGAAGTATTGTTGT11.1494252873563218No Hit
GCTGCGCATCTCAGGAGCTCCCATAACCTCTGGAGACCACCTCTGGTGGACTCCGGAACTAGCCCATCCCCTTGG11.1494252873563218No Hit
TATAAGCACCCTTACCTGGAAGACCGCGACGTTAAACGCGTGGGCTACCTGGTGGTGTCGACCGACCGTGGTTTG11.1494252873563218No Hit
ACCCGGAACTGAGCGGTGTTGGACTGGTGATCCTTGATGAATTTCATGAGCGCAGCTTGCAGGCGGATTTGGCGC11.1494252873563218No Hit
GGCTTTTGATGAGCGAACAGACATTTTTTATTTCCGCGTAATCCAAAGCATCAGCAAGGCATTATCGCATTATGA11.1494252873563218No Hit
GTTTAGGGAAGTTACTGAAGTTATAATAAATAAGGATAATACTATGCCTTCCAGGCATAGTAATGTGGATATTAG11.1494252873563218No Hit
CGCCAAAATTGCGCTAAATCAATCTCCCTTAAAGTTGCATGAAAAATCCCTTTTATCCCCGCGTTAAGCGTCTTA11.1494252873563218No Hit
CGCATGCCCCGCCCCCTCTACCTCGTGCCCTCGCATGCCCCGCCCCCTGTCTCTTATACACATCTGACGCGGCCG11.1494252873563218No Hit
AGCCAGATGTGCCAATCAGCACCGAAAAACTCCCTTCCTGAAAATTGAGATTGAGATCGTTAACGGCTTTTTGTG11.1494252873563218No Hit
GGCCTAGTCTCGGAGACCCTGCTTCCTAAGAGTCATCCATTACCTCTGGCTTTTAAAATCTTTCTGCCTCCTCTT11.1494252873563218No Hit
GGATAACCGGCGGTAGCTGCACGGCGGTGTAACCCATGCCGATGTAAGACGTGAAGCGTTTATTGCGACTGGCAA11.1494252873563218No Hit
GAGAAAAACATAATTTTCTCCCTTCACAATCAAGGCTGTTCCACTTCAACAATTGTGCTTTGCCCCATGACTGAC11.1494252873563218No Hit
GCTACAGTGTACCTTGTTATTAGTTATATTATTAAGTAATTATTTATTATATATATGTAAGTACACTGTAGCTCT11.1494252873563218No Hit
GGCCTGCTATCCCCGAAACGGGTTCAAATCTGCCCAGAAAAAGGGGGTTGTATCTTCGCCTGCCCTGTCTGTGTG11.1494252873563218No Hit
CTTTAAAGGCGTGGTGGCCTCTCGGTCGGCGCCTGCGCAGAGGTGGACTCGTGGTTACCCTCAGCCTTCGCCCCA11.1494252873563218No Hit
ATGTACTCCTGCAGCGTATGACCGTTGAGTGAAATAACCAGCGGCAGCGAATCGGTATGAATCAGTTCAACGGTA11.1494252873563218No Hit
AGCCTCAGTAGGTGCCTGTGGGGGAGCAGCATGGCTGGGTGTGTCTTCTGTTGCTAGGAGATACCCTGGAGTGAC11.1494252873563218No Hit
ATGTAGCAGAGAGTGGCCTTGTTGGGCATCAATAGGAGGAGAGACCCTTGGTCCTGTGGAGATTTGATGCCCCAG11.1494252873563218No Hit
AGTGTGAACATTGTAGCTAGTGTCCATTTCTGGGCAAGTGTGAGCATTGTAACTAGTGTGCATTTCTGGGCATGT11.1494252873563218No Hit
ATGCATGCCAACCATAGTAAGTTGTTAGATCATGAAGCGTCTAAGGTGTGTGTTGTGAATGATAAAATTATGAGG11.1494252873563218No Hit
ACCGCATGCTGCGCACGTGCCGCCATTACTGGGGTAAAGGTGACAGCGAGTGGAAAAAGAACCTGAACAGAGATA11.1494252873563218No Hit
GGCGGAGAGTGCCTTCGAGGATCAAAAACTCCAGTTGCTGCTCAATCACATCGGAGAGTTTTGGTTGGCGGATTT11.1494252873563218No Hit
AAGCATAGGTCTTCATAGTCAGTATATTCTTAGCTTCAGTATCATTGGTGCCCTATGGATTTAACGGGTAATACG11.1494252873563218No Hit
GTCCAAAATTAAAATAAGTGGACAACAAAGTCAAAAGGGGAAAAAACATCAATCAGGGCGAATGCCCACAAACTG11.1494252873563218No Hit
CCCTAGGGATAACAGCGCACTCCTATTTAAGAGTTCATATCGACAATTATGGTTTACGACCTCGATGTTGGATCA11.1494252873563218No Hit
AAACAAGACAGTAATTGCCAGCAGCCACCAGAAGTTAGGCTGTACGACGCTGGAAAGTAAAATAATCAAAAACAG11.1494252873563218No Hit
GGTAAAGGCTGCCATCTTGACGCACAGATGATTGCCGACGCCGCACCGCGTCTGCCACTGGACGATAACGGTATT11.1494252873563218No Hit
GGGCGCAGAGTCTGCAGACACCTGCAAAGTGCCCACAGGACCTGCCACAGGATCTTAAGACCTCTAGTGAGTAGA11.1494252873563218No Hit
ATGCGGAAGAGATAAGTGCTGAAAGCATCTAAGCACGAAACTTGCCCCGAGATGAGTTCTCCCTGACTCCTTGAG11.1494252873563218No Hit
AGTTAATGTAGCTTAATAACAAAGCAAAGCACTGAAAATGCTTAGATGGATAATTGTATCCCATAAACACAAAGG11.1494252873563218No Hit
GAATTTATCCACGCACCATAAAATGCCCGTCACCAGTGTGGCAACTGTCTCTTATACACATCTGACGCTGCCGAC11.1494252873563218No Hit
ACGCAAAGCCCATATTACGGGCGATCCCTGGCATCCGATTACCGTTACAATCCTGTTCATAGCAATCATTGCTGC11.1494252873563218No Hit
GAGTATGCGATTGACTATCAGGAAGGGATCAAGAATCCGGTAGGACTTTCGGGCGTGCGGATGCAGGCAAAAGTG11.1494252873563218No Hit
CTTGTCTCCCTGTGGACAGCGCACCTGGCAGCGATGGACTATCTGCGTCCGGGCGAGTATTGACATCTGACGCTG11.1494252873563218No Hit
GTTGTATGTACCCTAAGATTTTGCGTTCTACTAATGTTAGGAAGGCTATGGCGATTAGAATGGGGACGAGGAGTG11.1494252873563218No Hit
CTATCACTTCTAGGGACACTTATATTTCGCTCTCACCTAATATCCTGTCTCTTATACACATCTGACGCTGCCGAC11.1494252873563218No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAAG50.069.061
TAGAAGG50.069.062
TGTGACG50.069.09
TGTTGGT50.069.039
TGGTTCA50.069.042
GGCATTT50.069.01
GTCATCT50.069.022
GGCACAG50.069.049
ATCTTCC50.069.025
AGAAGGG50.069.063
ATTAGAA50.069.060
TTCCGGT50.069.028
AGGATCA50.069.054
TCAGGCA50.069.046
CATCTTC50.069.024
GAAGTGA50.069.015
CGAAGTG50.069.014
AGGGTGG50.069.066
GGATCAT50.069.055
GGTTCAG50.069.043