FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524493
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17960.3424259237015556No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATC4100.030.2975869
TATACTG854.4231165E-628.4109425
GTACACG500.007732241427.5992011
TATAAGT500.007732241427.5992014
GGTCTAC700.001333105624.6421431
CGATCTA1001.5596024E-524.15390469
GTATAGA1052.2782313E-522.9993321
TCGACAC750.00199192222.99933238
CAAAGGT1803.4197E-1022.9993324
GGCTAGA1352.6542693E-722.999331
CGAGCGA9850.022.06955769
GTTCTAG1103.2632764E-521.9539091
GAGCGAT5750.021.6034968
GTCTATA800.002897841221.5618741
TACTGTT1154.5975365E-520.999397
GTATTAG1004.3313246E-420.69941
ATACTGC1356.813416E-620.443856
AAAGGTG1409.329837E-619.7137155
ACTTGTG1056.018178E-419.7137138
TAGAAGT1258.7314256E-519.3194394
Produced by FastQC (version 0.11.5)