FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244359
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4980.20379850956993603No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAA252.563174E-455.21
TAGGTTG350.001345738239.428575
GAACTAT400.002592353234.56
CGATCGA501.8571435E-434.569
GCCTATG652.3140998E-531.8461531
CTACTCT553.249584E-431.3636384
GTATAAG703.838756E-529.57142612
TTAGGAC605.4098724E-428.753
TGTATAA605.4098724E-428.7511
TATAAGA854.4094268E-628.41176413
GAGCGAT2600.027.86538569
AGCGATC1503.45608E-1127.669
CTCTAGA500.007723227.62
ATAAGAG1101.1465763E-625.0909114
CCTTAGG700.001330845224.6428572
TCTTAAG700.001330845224.6428572
TAAGAGA1151.6885424E-624.015
CAGAACT1202.4447E-623.04
TAGGACA750.001988560423.04
CTGTGCG1202.4447E-623.09
Produced by FastQC (version 0.11.5)