FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25217
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCA200.0072762451.7500045
CAAAAGG200.0072762451.75000426
GCCTAAG200.0072762451.7500041
GGCTAGC200.0072762451.75000417
GAGCGAT350.001322908739.42857469
CGAGCGA350.001322908739.42857468
ACGAGCG450.00453829530.66666667
GCCGACG1150.001069818618.00000269
TGCCGAC1300.002431126815.92307768
CTGCCGA1708.6880365E-414.20588269
GCTGCCG1900.002011153612.71052668
CGCTGCC2100.004258629411.50000167
ACGCTGC2200.00602492110.97727366
TGACGCT2350.00983333110.27659564
GACGCTG2350.00983333110.27659565
Produced by FastQC (version 0.11.5)