FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGATGGAGTGGGGCATTCTGGGAAGCAAGAAGATAGCTATTGAGGACTTCAGTATCTAAAACCAGGATTGGATG11.2048192771084338No Hit
TTTCTGAGTTGCACACCTTGGAAAGTCGCGAGTAGGTTCTTCGTAGAGCAAAAGTGAGGCGTTTGCAGCGCCCAC11.2048192771084338No Hit
GTCCTGTAAAAGACTGAGAGCTCCGCCTCTCCGATACTCGCGGGCAGCCATTGCGGCGCACAGAACCGTCCGCGT11.2048192771084338No Hit
GACCTGGCCCTGCTGAGGCTGTGCGATGATGATCTGCCCAGGCCTGTCTCTTATACACATCTGACGCTGCCGACG11.2048192771084338No Hit
CTACAGCACAGCACAGCACAGCATTCATCCCTGCAGCACCCTCTAGATAACCACCACTCTGACACTCTATTACCT11.2048192771084338No Hit
CCCAGACAGCCACGCAGAACATACGCGGCAGTGCGACGCCTCCCCCACTGGGGACACGAGACCGCGAAAGCAACT11.2048192771084338No Hit
TGTCTGGACAGCAAGCTCACCTCTCCTGAAGGGATGCTTTTGATGCTGTCTCTTATACACATCTGACGCTGCCGA11.2048192771084338No Hit
CCACAAACCAACAAAACAGATAACTATATGACCACAGACCTGCTGCGTGTCATTAAAAAATCACAAACAACACGA11.2048192771084338No Hit
GAGCAGCTGTGTGTGTAGAGCTGACGCACGTCCTCTCCAACCCTTAGTGCTCCTGTGTGCTAATGAAGGATGCTG11.2048192771084338No Hit
GTGCTAGGGCAGGGACAGGGCAGGGACATGGTAACACAGGGCCAAAACAGGGCCAGGGTGGGGCCCTGTCTCTTA11.2048192771084338No Hit
GCCCTGGAACAGCTCTTACGAAGCAGAGGCACGCAGCTACCTGGCTCTCCCGAAAAAACTCTAAAAAACCAAACT11.2048192771084338No Hit
AGCCACTGCAAGGTGGAGGTCGGTAAGACTGAAATCTGTGGGCGCGTGGGGCTTTGAGGAGTAACCAGCCGTCGT11.2048192771084338No Hit
GGGTGGAGGAGAGTCGATGTCGGTTTTGGGGCGGGGCTGAACCAAGGCCCTGTCACTTATACACATCTGACGCTG11.2048192771084338No Hit
CTCCCACACAGTCCCTGCTCTGGATATCTGCACCAGATGGTGCGGGGATCAGGCAGGAGGCAGAGAGTAAAGATG11.2048192771084338No Hit
CACACACACACACACACACACACACTCACACACACAAAAGTGCGACTGTATTAGAAACACATCTGTCGAGGGCGG11.2048192771084338No Hit
ACTACCTTCAGCTGCCCACAGCTTGGCCCAGGTTGCCTCTGTCTCTTATACACATCTGTCGCTGCCGACGAGCGA11.2048192771084338No Hit
ACCCAGCACACAGCGAGAAAACACCGCCACAGAAGAAGGGAGGGGGATCCCCGTCCCTCCCCACCGACTCCCAAC11.2048192771084338No Hit
GTGCTGGGCCTGTGACATCTCTGGAGGTTCTGGGGTGGAGAAGGACCGGCGGTGGGGAGTGAGTGGCTGGGGGGT11.2048192771084338No Hit
GCATAACTCTGACTCCCAGGGCAATTAACTTAGAGTCTCTGTCTCTCAGTTTCTTCATGAAAAACATCGGGATAG11.2048192771084338No Hit
GGGTTGGGAATGGGAATTCCTGCCCGAGAGCGGAGTGGGGCCAGGCTGGGACTGTCTCTTATACACATCTGACGC11.2048192771084338No Hit
CCACTGTTGAGTACTCTCCAACAGTGCACATCCACACTAGGCCATAGCAACCCAGATATATCCAGAATCTTATAC11.2048192771084338No Hit
CTCCCACAGCTGGAGGTGCGGTCCCCGGTGTCCTTCCAGCAGGAGGAGAGACCGGGCTCTGTCTCTTATACACAA11.2048192771084338No Hit
AACATCTCAACCTCAAAAAACAATAATAAAAAAAAAATTGAAAGCTAAAAAAAAAAAACTAATAAAACACAATCC11.2048192771084338No Hit
GGTAGCTTGTCTCTATCAGGGGTGTGTTGGGCTCGGCTTTGACATCAAAAGTACAGAGCTGATCTTACTCTTTCA11.2048192771084338No Hit
AGGGCACACAGGCACTCTGGGTTGAGGCTCTGGTTTCGTTAAGAGGGCACCTCTTTAGTCTGGTGACCTTGATTT11.2048192771084338No Hit
GACTGAATGCTCACCTCACCTTTCCCTGCAGAGGGGTGGGGTGCGTGTGGGAAGGTCGGACAAGCTGCCCCAGGC11.2048192771084338No Hit
CCTCAATGCCCTGGGGTGCTCAGATGAGGAGGGGGCTGAGAAGGGGCTGTCTCTTATACACATCTGACGCTGCCG11.2048192771084338No Hit
TCCCGCGGCCGCTCAGCAAGGAAGGCGGGTGAGGGGCGGTGAGAGCCCGGTCTCTTATACACATCTGACGGGGCC11.2048192771084338No Hit
GCCTAAGGCTCGCTCCCCACCACCCGAGCCTGATATCACTCGCAGTGCCTAAGGGCTGTTCTTCACTCCACCGCC11.2048192771084338No Hit
CAACAACCCTGACTTGCCCTGCCACAGCCCACTCAGCTAACATCTCTGGGAAGGAAATCAATGACATCGTCCTAT11.2048192771084338No Hit
TCCAAATGCTGGCCCAGTTAGATACAATCTTAAAGCTGACCCACATCTTACAATTATACAAGTCTGAAACAGCAT11.2048192771084338No Hit
GTGTTTGTTCCCGCGTGCACGTGGGTGTCATCTGTGTGGCTCCGTGAGTGTGTGTATCCAGTAAATTATACACAT11.2048192771084338No Hit
CCATTTGTTTCACAGAACTCCACAACCGCCAAACAGCCAAGCAACAGACTCTCAATAATATAAACAACTTAAGAA11.2048192771084338No Hit
GCCGCGCCGAGGGGGCGGACGTGACTTGCATGCGAGCGGGACCCGGGCGCGCGGCGCTCGCGAGGAGGCGGCGGC11.2048192771084338No Hit
GCTCTGATCCGGGCCGTTTACACGTCGCGCTAACTCCGGAAGACTCGTAAGATGCTTAAACTCCCGTGCCCTGCA11.2048192771084338No Hit
AAATAAACTAAATAAAATAAAACCAAATAAAACATAAGAAAAATATAAACAAACACACACTAAAAAACAACACAC11.2048192771084338No Hit
GCATTCACCTCATCATGCAGGTGATCGAGATAGAGCTCACTCAGATCTCGGTTGTCGCTCGTCTCACCCGGGCCA11.2048192771084338No Hit
ATAAAAAAATCCAAACATATCATACCTCCAAAGGTAAATCCAAAATACCAATGACACAGACAAAATAACTGATAA11.2048192771084338No Hit
TCCTAGGGCTGCAGCTGCTGGCTGGCCCCAGGACGGCAGCTGCTAGGGGCTGCTTCCTCTCCTGTGGACGGGGTG11.2048192771084338No Hit
GCATTCATCACCCGTCACCGGAACGCCCTGCCATTGCCACACTGCCTGTCACTACTAAACTTCTCCCGCTGCCCC11.2048192771084338No Hit
GACTTGGCAGGTCTGTGGGGCCTGCACAGTCCATGTGGGGCTAAGGGACTACAGGGGCTCATGGAAACGCTGTGA11.2048192771084338No Hit
GCGCAGGTGAGTGATTGTGTGTTGGTGAGGTCCGCGGGGGGGGGCTGGTGCGGGGGCGTGGGCGGCGGGGTGGGG11.2048192771084338No Hit
CCCGCGGTCAATGCCCGAGGCAGCCTGAGCGCGGCTGGGGCTCGGAAAGCGCCAAGCAGGCAGGGACCGAGAGGG11.2048192771084338No Hit
ACTTTCACCCGTGTCTCATTGGCCACACCAAGTCACATGCTGTCTCTTATACACATCTGACGCTGCCGACGAGCG11.2048192771084338No Hit
ACTCAATATTTACCTGCAACACTCACATTGCGAAACAGGTAAATGCCTGTCTCTTATACACATCTGACGCTGCCG11.2048192771084338No Hit
CCTCCCAGTCGCAGACGCACCCGCTTTCCGCCCCGCGCGCCGGAAGTGGCCGAGTCCCGTCGGCCCCTGCGGGCC11.2048192771084338No Hit
AATCTATAAAAAGCAACATATCCTCGCTAGCCCAACAGCTGCATCACAAGACTCTAATACACAACTGACGCTGAA11.2048192771084338No Hit
GCACTCACCAGTCCAGTCCCACGCAACAAAGAACTGCGGATATGGGCACATGCAACTTACACGTGCACACAGCCG11.2048192771084338No Hit
CTCAAGTAGATGAATGCAACATACAAAAAAACAATCTATAATTGAAATGATCACACTAAAAAAAATAACACGCTT11.2048192771084338No Hit
ATGTGACATCTCTCAGTCATGTGGTCATCAGCAGCTGCTCGGAGTAGAGCCTGTCTCTTATACACATCTGACGCT11.2048192771084338No Hit
CTAGAGTAGTGTCTAATATTTTAAAAATTATAATATCTAAGTAATATGTATCCCATACTGAAATGAAGAGCTGAT11.2048192771084338No Hit
CGCCAGCTGAGGCCGCTGCAAATCCCTGGCCTGCAGCCCCCAGACTGTTGCCCCTCTCCAGCCTCAGAGCCGCCC11.2048192771084338No Hit
CTGCACGCATCATCCCATTTGATTCCCACATCAGACCGGTAAAATAAGAAGTGAGAGGTGCTTAGTCCATTTTAC11.2048192771084338No Hit
CTGCAATGTCACTCCCTCATCCTGCCACCAGGTGGCAGTGCACTGCTGATCATTCTGTCTCTTATACACATCTGA11.2048192771084338No Hit
GTCCTGAACTGGCACTCTCCAGCGCTCTGGTTCCACAGGGACTGAGAACACCCTGTCTCTTATACACATCTGACG11.2048192771084338No Hit
TGCCCAATAACAGGAATTAGAGGAACAGATAAAATTGACAAAGGGTCAAGACTAAATCCAAATGAGTCACCCAGG11.2048192771084338No Hit
AAACTAAACTCGCCGAGGAAAGATAAGGGGGAGGGGCTGACGTTGAGAAAGCTGGAGGGCAGGGAAATGGGCCGA11.2048192771084338No Hit
CATTAATGCATGCCACCTACTCATGCTCCTAATTGTAATCGCCACCCTATCAACTGACAATTAAACACAACTGAC11.2048192771084338No Hit
AAATTGAAAATCGAGTCACTAGTCCAAACAAAAACCCTAAAGCATTCAATTAACATATACCCTGTACAATGAACC11.2048192771084338No Hit
GGCCTGCGTAGAGAGAAGCGGAGCGGGGCGTCCACGCCTTGGGGAGGGAAGGGCGTCCCCAGCGGGAGAGAGTGG11.2048192771084338No Hit
CCACACAGGGGACCCACAGAAAAGCTGTTTCCGCTGGTATCGGCCTGTCTCTTATACACATCGGACGCTGCCGAC11.2048192771084338No Hit
TCTGTACCCACGCCTTCTTCAAAGCCATACTATTTAAGAGCTCCGGGACCATCATCCACAACCTGAACAATGAAC11.2048192771084338No Hit
CCCCGGCATCGCTCTACAAGAAATTACCACCGGCCCGCAACGACAAAAGGACAAACGGCTACTTCACAAAACCAC11.2048192771084338No Hit
TCCCTACCCTTCGGGGCTGCAGTCTATGTCAATCCATGAGTCCTTCTGAATGCTCTTGGGGTCTTCCAAGGCTCT11.2048192771084338No Hit
GACATAACAGGCTGATCATTTGCCTATGGTTCCTATAGTTATAGACTGTCTCTTATACACATCTGACGCTGCCGA11.2048192771084338No Hit
AATTAGCATTTCCAGCCCCCATGTGAAGCCAGATAATGGAAGGGAGAGCAATTAAATAGACGCTGTCTCTTATAC11.2048192771084338No Hit
GTCATAATCCTGGCCTCCCTCTCCCAAGTGTGATGGTGGAGAGCAAATGAGCTAAGGTTTCAAAGACAATCCCAG11.2048192771084338No Hit
CTAAGGAAAAACGCAGAAATTAAACTCACCAAGAGCATAATCAGACAACATAGAGGACGACTAACCAAACGTGTT11.2048192771084338No Hit
CGATAAAAAAGTGAGCTTTAGAGGACCCGAGTTTTAGACCTCTCAAGAAGTGATCTTTGACAAAAAGATAGCAGA11.2048192771084338No Hit
TTGCTGAGGAGCTCCCTGGAGGTCCGTGCTGAGGCGACGCGGCGACCGTTCTGCCTGGAGACTGCGGCAGCGCTC11.2048192771084338No Hit
GCCCGGGGGTTCCCTAGGGGAAGCAGGGGCTGCGGCAGACGCCTCTCGGGCAGGTCAGGGTCTGTCTCTTATACA11.2048192771084338No Hit
GACTAAGGGCGTCCTGCCTGGGCTTGAAGCTGCAGCCCTGTTTCCCCTCCGCATTCCCTGTCTCTTATACACATC11.2048192771084338No Hit
GAATCTAATGCTGTCTCACACACTGGAGTACCTTCCCCAGATATCTTCTCAAATCTCACTCAGTAGCAGATTCCC11.2048192771084338No Hit
GAAGAGAGATGATTGAAAGTGATAAAAAGAAAGATTATCATAATGCATCATCTTTTCTCTATGAACAACATTAAT11.2048192771084338No Hit
GCTAAACAAAGCCCTCTAAGCTCTCAGGCTCATCACCATTCCTACGCCATACTAAGATCAACTCGGACGGAAGAC11.2048192771084338No Hit
ACCCTAAACACAGAATCCAACACACACTACAACAAAGAAATGAACAGACACCACCAAACCATCCAATGCATCACA11.2048192771084338No Hit
GTGGGGGTGCCGGTGGTATATAGAGGTATAGATTAAGGAACGTGGGCTAACTCTAACCGGGTGTTTATGAGCTTA11.2048192771084338No Hit
GCCAAGGCCTCTCCAACTGCGTCTAACACAATGAACACCAGCAAATTCGTATTAACATAGAACTCTTACGCATAG11.2048192771084338No Hit
CTGTTGACCCGTAAATGGGGGATGGGGCTGGCCAGGGTGGAAAAGGCTTGTCAGACCCTGTGCCAGGACCTCATC11.2048192771084338No Hit
CAAAAATTCCCCCACCGTACTCACAAGAAATGCTTCTAAACTTCCCACCATCAATATCCTTAACAAATTATAAAT11.2048192771084338No Hit
GGTCAAGGGACACCTCTCTCAGGGGTGTCATACATATGGACGAGAAGCTATTTGACTGTAATATGCTATTTAAGC11.2048192771084338No Hit
AATCAGCCGCGTCCCAGCCACTCCGAGCAAGCAAGGTAGACCCACCGAATGTCAAAAATACACATCACCCGCACC11.2048192771084338No Hit
CAGCCAGTTTGCCAGCTCATGCACACGCTCACGCTAACGCACAAACACAATAGAGCGGGTCGCTTCCCGCTGTTT11.2048192771084338No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACAGC50.069.01
ACAGCAT50.069.018
CTCTGAC50.069.057
TTCATCC50.069.024
CAGCATT50.069.019
CAGCACC50.069.034
TCTAGAT50.069.042
TACAGCA50.069.02
ACCACTC50.069.053
CCTGCAG50.069.030
ACCACCA50.069.050
CATCCCT50.069.026
TGACACT50.069.060
GATAACC50.069.046
ACACTCT50.069.062
ACTCTGA50.069.056
ACTCTAT50.069.064
CATTCAT50.069.022
TCTATTA50.069.066
CTGACAC50.069.059
Produced by FastQC (version 0.11.5)