FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences658977
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13300.2018279848917337No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG2050.047.12386712
TATAAGA2550.037.88389213
AGCGATC6200.034.50402569
TGTATAA2850.033.89611411
GTGTATA3050.031.67341810
TCCTACG553.254755E-431.364912
ATAAGAG3350.029.86688614
ATGTGTA3300.029.2716988
TAAGAGA3750.027.60112415
GAGCGAT9650.027.17415269
GATGTGT3750.026.6790627
CGCCGTT809.5531155E-525.87605312
CGATCTA1759.094947E-1225.6315669
AAGAGAC4200.024.6438616
TAGTCCG700.00133292724.643865
TAGGGTA1001.5618545E-524.1509845
TGTGTAT4000.024.1509849
CTCAGAT4300.023.2683893
TCAGATG4250.022.7303374
AGATGTG4450.022.4840626
Produced by FastQC (version 0.11.5)