FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTAGTCAGAGCGAGACGTCTTTACAGGTTTGGGCCGGCAGCTGGAAAAGGCACACGGCCCCGGGCTCCTCCT12.0No Hit
CCCCACCCCCATCCCACAACCACCCACCTGCAGCGGCCCTGCAGCTGTCTCTTATACACAACTGACGCTGCCGAC12.0No Hit
GTACATCCCTGAGTGGGCGGGGCTCGGCGGGGCTCTCCGCGGGGTCCTAGTGCTGTCTCTTATACACATCTGACG12.0No Hit
CCTAATCGTCCTTCCCTAGGGGACCGGTTAGAGCAACTGTAGGACCTGGCTCTTATACACATCTGACGCTGCCGA12.0No Hit
GAATTACACACATATACTGGTTTTTTTGATGGGAACCTCCAATCACTGCTGCTTCTTCTTGGCCATCTTTGTCTC12.0No Hit
TTTTTAAGCTAAGTAAAGGGCTTTTTCTGCGACTTACGTTAAGAACTGTCTCTTATACACATCTGACGCTGCCGA12.0No Hit
AAGTATAACTGCGAGCCCCAAGGCGTCACTAGCATGGGCATCCCAGCCTCGGTGCTTCAAGTCCTACTTCTGCTT12.0No Hit
GCACCACAAACAACCCAAATAAATTATGTACAAACACCACTAAGCCTGGCTAGTATAAACAACTGACGGTGCAGA12.0No Hit
GCATGTCGTCCACAGACCAAACGGGATCGACATCAACTTCAAGACCGGCACCACGGACAAACATGACGCTGCACT12.0No Hit
GCCACACTCCGATTCCCTCCTCAAACCCCTCCCCGTTTCCCACACCCTGGACCCCTCGCTCCGTCTCGGCCCCGC12.0No Hit
ACTAGTAAGACTGCTGCAGAGTCCGGAGGAAGAAGTCACCTAGAAAAGTCTGGGACAGGGCAGTAAGCTTCCTTC12.0No Hit
CTCCAGAGTATTCGACGGTTCATCCAGGTTTGAGCCCCACTTCTTTAAGACAGATATCGCCACACAACCCTGACC12.0No Hit
CCCTTCCACCCACTTCTCTTCATCATCCCCCCTCCCTCTGCTCTTTCTATTCTCTGTCTCTTATACACATCTGAC12.0No Hit
CGTGTACTCAGACTGATCGGGGAGGAGGAATCTGACAAAAGGAAAACAGCAGCTCTAAACGATCGGATTAGGGGG12.0No Hit
GGCCGGGGCGCGGGGCTGCGCGGGCGCGTGGGAGGGGGGGGGGGCGGGCGGGGGGGGGGGGGGGGGGGGGGGGGG12.0No Hit
ACTTTGACTTGCCGAACATGTCAGCAGGTCATTCAGCATTCAGGAGAGAAGGAAAGGGAGTTCCCTGGCCAGCTG12.0No Hit
GGAAAAAACATTTTAGGTCCTCAATAAATATGTTGAAAAAATAGGATATTAATTTATAAGACAATCAAAATAGTA12.0No Hit
CACACACACACACACACACTCTCTCTCTCTCTCTCTATGTTACTCGTAAAGAAATGTAAATTAAAACACAAAGTC12.0No Hit
GGCATGCACTGAGAGGTAGGTCAATCAAAAAAAGTATTTGATATGAATCAAAAACACATCTAAAGCTTACGACTA12.0No Hit
CCATGATTCCACTCTCTGACCACCACCTTCCTTCTTTCCTACTCACTCCTTACACTGTCTCTTATACACATCTGA12.0No Hit
AGATAGGGGTCCCTTGACCACCATCCTCCGTGAAATCAATATCCCGCACAAGAGTGCTACTCTCCTCGCTCCGGG12.0No Hit
GCCTGGCCCTGCATCTTAATGGGGCGGTGAGCTCACAAGATGGATTTAGCTGGGGGTTTTATGGTTGCCGCGGCG12.0No Hit
ACCCTAGAACGACGAGGCTGGGACCCACCCTAGAAACAGGCTCTTATACACATCTGACGCTGCCTACTATCCATC12.0No Hit
GCGAAAAATACACCTCGTAAGAAAAAGCATGCGGGACAAACTTGCTCTTAACTTAACAAAACAGCCCGACTTCAA12.0No Hit
CCCCTCCCCCGCGCCACCGGGCGGGGCCGGGGGCTGCTCGGCCTGGCGCTTATACACTTCTGACTCTGCCGTCGA12.0No Hit
ATGCAACGCGACCTCCTTCCCACAGCAACGAAAGACTGCGACTCCCCGGACTATCAGGCCGGGAGAGTCCACTTC12.0No Hit
CCCGTACTCTGACTGGGATAAAGAGCAACTTCAGGACGCAAACTCAAGCTGGATGGTCGAAGACTCTTTCCCGAG12.0No Hit
GCTTTATTCAGCACCAGAATGGTTGAACTCTGCTCCGGCAAGCGCCAGGCAACGCGTCCCTGTCCATTGCCTGTC12.0No Hit
GGTGTGGCCTCTGAACCTCGCCCTTCCACGTCCAGCTGCTTCGAACCCAGAAACCTGCACCTGCACCGGGGCCTA12.0No Hit
CTGCAGGCCCACCCTCATCTTCTGCAGAGACTCCCCTAGACACAGACTTGTACCTGTCTCTTATACACATCTGAC12.0No Hit
ATTCTCGTCCACTGGAGGCCAAAGCCTGGGAACTAGAGCAAGCGGCTGTCTCTTATACACATCTGACGCTGCCGA12.0No Hit
GATCAATGCCTTACGTTTTTACCCGTACAACCGCCAGACAGCGATCTTCCTTGAGAGACAACCACCTGAACCGCC12.0No Hit
ACAATGGGCGCTTGATGGCTGGCGCGACTTCTTCAATCGGGCAAACGGAAACCGTCGAGAGAGTAAACGCAATAC12.0No Hit
CCCCATACTAGTTATTATCGAAACCATCAGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAA12.0No Hit
CCTAATCCACATCCCATACGACATAAACAACATCCAAAATTAATCCTGTCACTTAAAAAAAACACACCCACCCGA12.0No Hit
GCCTTGGACATTGGCCGCGATGCCCAGCACAGCCCAGGGTCTGTCTCTTATACACATCTGACGCTGCCGACGAGC12.0No Hit
TGTAACTCTTGATAATTCGCTTCTGTATTGTAAATTTAGTGAAAATATAAGGTTAATAAAAATGGACTAACTAAT12.0No Hit
GACACTAACAGAAACAATTCAACAAGAAAACCCACACCAAAACCAATCACTCTTATAAACATAACACGATTCCAA12.0No Hit
GCCTTATTAATTTTAGCCAGAAGCTGGAAACAACCCGGGTGACCCTCAACAGAGTAATGGTTACAGAAAATGTGG12.0No Hit
CCGCCGCGCCGCCCGCCGCCTACGGCCAGCCGTGCGCTCAGGGCCTGGAGGCCGGGGCCGCCGCTGTCTCTTATA12.0No Hit
CGATACCACAGCGCACACCACATCTCAATGAAGCTACTTATGCTATAAATAAAGACGACCATACGAGAAATCCCA12.0No Hit
CTAACCACCCACGTGAACCCAAACAAACAAAACTCCAACCCCCAATATGTAAAAACCATTCTCGCATCCACCGTG12.0No Hit
CCGCTGGGGTGCTGTCGGTAGGCGGGCGCGTGTGGGTGTGTGGCGGGGGGGTGGGGGGGGGGCGGGTCGGCTGGG12.0No Hit
CCCAAGCACAGTCAAGCTTCTATACTGACTTGACACATCACCATAAATCCACTCTTTCACGAAACATGAATCTGA12.0No Hit
GTTTCTATCTGGTTCAGTCTTTGTAGGTTGTATGTGTCCAAGAATTTATCTATTTCCTCTAGGTTTTCCAGTTAG12.0No Hit
GTTGCAGTGAGCCAAGATTGTGCCACTGAACTCCAGCCTAGGTGACAGAGCAGGACTCCATCTCAAAACAAACAA12.0No Hit
CCTTCACCCCTCCTCTACTACTCCCACTCCCAATTAATAACAAAACAACACAGACTGATAAGAGTTTTAAGACGA12.0No Hit
GGCCGGCGGGGCCTCCCGGGAGGAGAACGGGGCGGTGAAGCCGCTGCCCCGAGGGGGGTGCCTGTCTCTTATTCA12.0No Hit
GCGTAGAAGTGAAGTACTTTTTTATTTGCAGACCTGGGCCGATGCCGCTTTAAAAAACGCGAGGGGCTCTATGCA12.0No Hit
GCATATACCAGTAAAAGAATACATCTCCGACATTGATGTAGAGAGAATAGAAGGCCACGCCTGAACAAATCAGTG12.0No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGC50.069.021
CTCTGCC50.069.064
ACCGGGC50.069.016
TATACAC50.069.051
GGCGCTT50.069.045
TGCCGTC50.069.067
TGACTCT50.069.061
CTCCCCC50.069.04
CGGGGGC50.069.028
CGGGGCC50.069.022
GGCCGGG50.069.025
TCGGCCT50.069.038
CACCGGG50.069.015
CGCTTAT50.069.047
ACACTTC50.069.054
CGCCACC50.069.012
TTCTGAC50.069.058
CTGACTC50.069.060
ACTCTGC50.069.063
GGCTGCT50.069.032