FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAATACCCAGTACACGAAATACTTGTTATACCTCGATTTATAACCGCGAAGTGAAGTAACAAAGCTTGTTGATG11.1764705882352942No Hit
GTTTATGTCATACCGAACTACATGTTAAGAAAGGCTATTATGGTGACAAAACCCGCGTAAATCTGGGCCATGAAG11.1764705882352942No Hit
GGTATCAATGGTGTACCCCACAAGCACCGAGACCAAACCGGGGGGGGTCAAGGCTCTTGAGGGTATAGACGTCAG11.1764705882352942No Hit
CCTCTATAACCGCAAACAGACCGCCGAAGCAGTTCCCGCACACATGCCGGATCTGATCTTAATTAGAGCAACCGG11.1764705882352942No Hit
AAACATAACAGGATAATTATTATGGAAAACAACATCAAAACGTACGTTCTGGGGCTCAAGTATAAAAATTAAGAC11.1764705882352942No Hit
CTCCTCGACTGGATGTTACCTGGCGGCTCCGGTATCCAGTTCATCAAACACCTCAAGCTGTCTCTTATACACATC11.1764705882352942No Hit
GACTACGATTGCCTCGACGATAATTATTGAGGCTGTATAGAGTGTGACGACGTATTGTGTTGCAGCTTCAGTTGA11.1764705882352942No Hit
GCACTCAAAACACTCAATATGAAACTGATAGAAACAAGCTGTATAAAAAAATCAATGCACCTGACAATTATAAAC11.1764705882352942No Hit
AGGTAACCCCACCCCTGAAGGGCAGGGTTGATGTCGAATTACTGGCCTTTGTTTTCCAGATTCTCCACCTGCGGC11.1764705882352942No Hit
ACGTTAGCCTGGCAATCGGTGACACTCTGGGGACCTCTTACAAAAGTGGCATGGGTATCGACCAGAAAATCGTGA11.1764705882352942No Hit
GTATTAGCCATCAAAAAAATACAATCAATCCTATAAACATACACAAAACTCAAAAACAACAAAAATCACCATAAT11.1764705882352942No Hit
ATGAATTCAAATACCAAGATTCATTAAACACCCATAAATCAGTAAAATATACAAAAAACACAAATCACACTACCG11.1764705882352942No Hit
CACTATATTATAGACTAATTCAACAACCCACATTAACACTTACATACCATACGGCATTAATCTTAGACACAACTG11.1764705882352942No Hit
GAATATTACTATAAAAATTACTCAAAATCTGATTCAGCCTGTACATCAACATAATACCCCTGTCACTATAACACA11.1764705882352942No Hit
CCTTTATGCACCGGACCTGTGATCAGCGCGGCAAATTCGACGTTCAGACAACCAGAGCACGCTCGCGCCATCGGT11.1764705882352942No Hit
CGCCTCATTCGGGCATTCGGGTTCACACATATCACAATTGATGCATTTTTTAGTGATTAACAACGCCATAAGGAA11.1764705882352942No Hit
GTACTCAACCAAGTCATTCTGAGAAAAATGAATTCGGCGACAGAGTTGCTCTTGCCCTGCGTCAACACGGGATAA11.1764705882352942No Hit
CCATTAGGGCAAGGGCTATTGAATGGATATGATCCTTTTCTTTCTCCTCGGTTAGGTGCTGTTGAGAAATATCTG11.1764705882352942No Hit
TCTCAGAGCCTGAGCAAAAAAATCACGTTACCAAACAATGACCAGGAAATCCAGGATAGACAAAAGAAATAGCCG11.1764705882352942No Hit
GAAAAAAGCAGTACCATAATATCGAAAACCGGTCCAATTGCTGAACAATCATCAATTCTTCGCCATACGGAACTA11.1764705882352942No Hit
GGTTAGTGATAATACCAATTCACGCTTGCGGGCCTGTTATCGCAACTTGCTGTACCAGTTTTTACACGTTCCCGG11.1764705882352942No Hit
CTCCACTAATTGTCTGATCCGTACTTATTACAGACGTACTGCTCCTATTATCACTACCAGTGCTAGCCGCACTCA11.1764705882352942No Hit
ACCCAGAAGTTTATATTCTTATCCTCCCAGGATTTGGAATTATTTCACATGTAGTTACGTACCTGTCTCTTATAC11.1764705882352942No Hit
ACCCTGGCGAAAGTTCTCTGACGCATTTGCCAGACGCTGGAGAAAAACGACGTTCTTGAGGCTCTGTCTCTTATA11.1764705882352942No Hit
AATAGGAACTGCGCTACACGCGAACTCAGAGGGACTCTTCCTGGGCTGTCCGTGGCTCTCTGGGGGTCTGTGCGC11.1764705882352942No Hit
GTTCAGCACTATCTGCCGCTTAATCCACAGCAGTTGCCAGGGCTGTCGTGGGATCTGGCGCTGAATACCTGTCTC11.1764705882352942No Hit
GACTAGAGTAGAAACTAATTAAAACAATTCAAAAATAAATACAAAAACATATAAATCAAATAAATGATAAATATT11.1764705882352942No Hit
CGGGGCGACTATCCTTAACCTGCGTGAGCAGCTGGGGCTGGATGGCGAACTGGTCAGCGACTGCGCGGTGCTGAC11.1764705882352942No Hit
GCCTATTGCACGCCACTGTGTTTATACTTAACACAAGATAAAACGGCAACAGCCAGTCTCTAAAACACATCAGAC11.1764705882352942No Hit
ATGTGCAGTGGGATCCCTTGAGTTACTTCTGGTAATCAGAAGTGGAATGGGGCGAGGCCTAGTTTTATGGATAGG11.1764705882352942No Hit
GGGATGGGGCCCGCTGCGTCACGCTAACGGTGAAATCGCAACCCGTGCTATCTCTGTCGCTTCTGCACATCTGAC11.1764705882352942No Hit
CTGACGAAGAGTCGGGAATACTTGCCCCGCACCTTGTCGTGGAAGCCGACGATAACCATCTACAAGCAATCTAGC11.1764705882352942No Hit
CTAAAATTAATAAGCGGTCAAGCAACTGCGCTACCCATCCCAAAATTTATTCCACGATTGAAGGAAAAATAAAGC11.1764705882352942No Hit
AACCTATCTCGGGATATAAAATTGCTTAATAACTGATTATGCCTAATTCGGCCTAATCTTAAAAAAATCAGCTGA11.1764705882352942No Hit
ATATAGGATATGAGATTGGCTTGAAAACAATTTTAGGTGGTTCGATTCCTTCCTTTCTTATTTTACATATACATA11.1764705882352942No Hit
GCGAAGCAACGGCCCGGAGGGTGTCGGGCAGGACGCCCCCCATAAACTGCCATGAAGAAAATCCCCAGACATCCA11.1764705882352942No Hit
GGATGAAGACAGTAACCAACCCGCACTGCATCCACATGGCGATAGACCTGTCTCTTATACACATCTGACGCTGCC11.1764705882352942No Hit
GTAACATACTAAAGAACTTAACTAAACTTAAAAACACCGATTAATAATACTCAACAAGACTCTAAAAAAAAAAAA11.1764705882352942No Hit
GTTGTGAAACCCGCACCATCTGACGATAGACTCACAGCAACACAAGTCTCGAATCTTTATTCATCTGCAGGCCAA11.1764705882352942No Hit
GTGCTGTGCCGCGCTGCGCAGGTTGCGGACTGAGTAGCGGCTGTCTCGTATACACATATGACGCTGCCGACGAGC11.1764705882352942No Hit
CCTAAATAAAACACCAGTAACACTTGCACATGACGATTACCTTAAACTAAAAATTTAAATCATCCGAATAAGTAT11.1764705882352942No Hit
TATAAAGACTGACTAAGCATGTAGTACCGAGTATGTAGGAATTTCGGACGCTGTTGCAACTCCCGCCAGCTCCAC11.1764705882352942No Hit
CCTTAACTCCCAGCTACTCAGGAGGCTGAGGCAGGACCCTTATATTAAACACAACTGACTCACCCTACAAACTAA11.1764705882352942No Hit
GGTACCCGGTAACGATCGACAGTATCACAAGAATGCTCTGGTTTTAATTGGGATTCATAACGGCGGAGAAAGTGG11.1764705882352942No Hit
GTTCAGTGCACCCCGTAGGCCTGATAAGTCGCGTCAGCGGCGCATCAGGCATCGGTTGTCGGATGCGACGCTATG11.1764705882352942No Hit
CTGTAGAATCAAACTAACAAAATTCACCCAATATAGCAAAAAAGCAAAACAGCTCTCATTCAGAATAAAATAATG11.1764705882352942No Hit
GTATAAGCCGTGCGCCGAACAAATCTATACAGCCAGTCTTTCGACGCAATTCATTTCGACATTTAGCGTGTCGAC11.1764705882352942No Hit
CTCTAGGGGCTGGAGGACCGAATCACAGGTTCTTCCGGAAGGCGCCGCTCTGCAAGGCCGAGGGTGCGGAGGAGA11.1764705882352942No Hit
GGACTGTGATGTGAATGATAAAATTAAGAGTTTTATGGTTACAACAAGGTTTTGGATAAAGATCAGGGCAATTAC11.1764705882352942No Hit
TCATATATGCGAGATGTGACACCGGTCGTCACCTGGTTAGCGGAGGCAATACGGTCAAGACCGCCGTAAGTCCGG11.1764705882352942No Hit
ATTATATATAATATGCGTATACAGGGGAGACCAAATATGATAAGTAATAAATCTGGTAGAATAGATCAAAAGCTT11.1764705882352942No Hit
CCCTAGACTTATAATCAATCTAAGTTGTCCGTAACCCATCAAACTATCTCTATGATTCACATATTAACATCACAA11.1764705882352942No Hit
GTTATCCACCACGCCTTTCTGCGGAATGCTAATCTGATAGCCGCCCAGTTCCGGCGGGTTTTTCAGGTGTTCATA11.1764705882352942No Hit
TATCTAACCCATTCAAAACATCACCAACGTATTAATCCCAGCATACATAATCACATTTCACAAATTATCTCCCAA11.1764705882352942No Hit
AATTTAACCTTTCTTCCAACAATCAGTAACAAAACATAAGATAAGAAAAACAGGGCATTAAAACTGTCTCGAATA11.1764705882352942No Hit
AATATGAAAAAAAAAAAAAACACAATTACTATTAAAATATAAACTCTATCAAAGAAACAATTATAGAAAATAAGT11.1764705882352942No Hit
CCATAGGCCATGAGTTATTGGAAATCTAAAAATAAAAGTGAACAACGGCGATTATTAAAAAAATCCAAATAATAA11.1764705882352942No Hit
GACATGCTGCGCCATATTTCACCCAGTATACGTAACTGTACGAAGCCAGTATCTTATAAAAAACACACGCTGAAA11.1764705882352942No Hit
GGGGTGTGGCCCCCCATTTAGCCCTTCGTGATCCATAGTCATCTCTACAAGTCATCTGACCTCCTCGTGCATACG11.1764705882352942No Hit
ATCTTACACTATTCCTGATTACCATGCTTATCCTCACCTCAGCCAACAACATATTTCAACTTTTCATTGGCTGAG11.1764705882352942No Hit
AACCAGCCCAGTATATCCGCCCGCTCATAAACACTCACCTTACCCTCCCAGAGAACCGACAGTCAAATAAACAAA11.1764705882352942No Hit
GGCTACGCAGATGAATACGGACGGAATAGCGGTAACTTTATCTCCGTGTCTCATCCATACATCGAGCAGCCTATT11.1764705882352942No Hit
GCGGGGGGGGCGGCGCTGGCGGTGGTTGTTATCGGCTAGAGTGGGCGCGCTGGAGCGCGTGTTCGACACGTGTCT11.1764705882352942No Hit
CTTGAAAAATAAACGAGAGTAAATTTACCAAACTCTAAGCGATTTATCCATCTAGTTAGCTAGTTGAACCCGAGG11.1764705882352942No Hit
CAAAAAACCTTTCTGTATTATTGAAAATAAAAAAGAAGATTCAGCATTCGATTTAATGTCTCATAAAAAAATCTG11.1764705882352942No Hit
ATCTTCGCCTTCTTCCGGTTTATTGTGTTTTAACCACCTGCCCGTAAACCTGGAGAACCATCGCGTGTTTCAAAA11.1764705882352942No Hit
CCGCACAGCATCACATGGCAGGTTTCTGTATTCATCGGCAGGCCGATCGCGCTTGCCAGTTCCCCACTTTCTATT11.1764705882352942No Hit
CCCCAAAAAAACAAACCAAAAAAAATCCAAAAAAAACAACAACATACATCATACAAACAACAAAACCAAAAAAAA11.1764705882352942No Hit
GGACAGCTGTGTGTTGCTGACGGTTTAAGGATCAAAGATGAGTACAATTTAACCTCCTGCCCAGTCGAAACCGCC11.1764705882352942No Hit
CCGTATTACAGGTGCGGTGATAATTGAAAACCATGTGGAGTTAACCTACCACGCCGTCGTTGAAGCCTTTGATGG11.1764705882352942No Hit
CCTAAAGGCTGGCGAAGAGTTATGCAATTTTGATGACCTTGCCCGCCGCGAGAGTGGGGGGTGTTCTGGTGGGTT11.1764705882352942No Hit
TCTCAAATACTCGCCGAAGGATTGATGAGGTCAGTTACAGAATGAGAACTAAACCTACTACCTGTATAAATCGCA11.1764705882352942No Hit
CAGTAATGCCGATTAACGCTTGCACCCTCCGTATGAAGGACGCTGAAGCCACGGAGTTAGCCGCTGCGGCTTCTG11.1764705882352942No Hit
GGTTGCTGGAATCAAAAAAAACATTACAAATAATGAAAAGATGCTTCCGAATTATAGGATATTAAATATTATAAT11.1764705882352942No Hit
GGTGTAAACCTACGCATAAAATCTCATAAGCTCGATCCGGAACAACTGACAGAGCAAACAAAACATCCACCATAT11.1764705882352942No Hit
ATCCACAAGTACCTAACGAGGTAACTTAACCATTTTACAAAAGTATGCTTATATTTATAACAACAACCTTAAACG11.1764705882352942No Hit
ATATTAAAGCATCTGGCCTACACCCAGAAGATTTCATGACCAATGAACACTCTGAACTAATCCTGTCTCTTATAC11.1764705882352942No Hit
GAGCGAATTCATAATGATGGATATATAAAAGGGGAGCAGCGCATTCTTCGATTGTTGTTGCAGAATGGGGCGGAT11.1764705882352942No Hit
CCTTAGAGGGGCGATAAAACATTTTGAACCGTGATTTAAATACCAGCGTCAACTCGTTAGCATAAACATCATCTG11.1764705882352942No Hit
CTTATCAAAAACTCCGAAAAAAACAAAAAACCGCTGCATAACCTCACTCTTCTCCTGTAAAGAATCACCCTACGA11.1764705882352942No Hit
TCAAAATCCACTCCAGATTCTTAGCAAAATACAATTCTCCATGAGCTGTAGAGAATACACATATGACGATGACGC11.1764705882352942No Hit
CAAAAGAACTTTGCGTCTCACCGTCCCGTAGCGTTCAGGTCAAGACGAAGGAACGTAACCACAAGAGACGCTGCC11.1764705882352942No Hit
GATGCGGTAATGGCAGCGTAGGCTCCAGGTTAAAGCGCAATCGTTTTTGTTGCGCCTGCAAGGCGGAGAGATTTT11.1764705882352942No Hit
ATTTACTGCCGGGTTTTTCGTGAATCAACGTAATATGTCAATAATTAATTCCACGCCAGTGCGTGTTATTGCCAT11.1764705882352942No Hit
TCTCAACACCACGCCACAATAACAGACTGGCATCCGGATGCCCCTGCGTCTGTTGCAGACAATACGCCCAGGTTT11.1764705882352942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGT50.069.027
AGCCTAT50.069.068
GTGTCTC50.069.046
CCGTGTC50.069.044
CTTTATC50.069.036
ATCCATA50.069.053
ATCTCCG50.069.040
GCCTATT50.069.069
TGTCTCA50.069.047
ACGCAGA50.069.05
TTATCTC50.069.038
TACGGAC50.069.016
CATCCAT50.069.052
GCGGTAA50.069.029
GACGGAA50.069.020
ATGAATA50.069.011
CGGACGG50.069.018
ACATCGA50.069.059
TACATCG50.069.058
GATGAAT50.069.010
Produced by FastQC (version 0.11.5)