FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259010
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGTT200.007351005451.75289532
GTAAGCC450.00461488530.6683853
ACGAGTC450.00461924730.66246429
GCCTTAG500.007714654327.6068761
CAGTACT756.143033E-527.6015454
AGGGCCG500.007721930327.6015455
GGACTGC1254.118192E-927.6015436
CCTTAGG658.6263544E-426.5450732
ATAGACT1057.6413016E-726.2871864
GCTTAAG809.51768E-525.8814451
TGGACTA1251.2559576E-724.8413895
CCGGGGT700.001330625624.6442384
GGGGTTC700.001330625624.6442386
TGTACTG1052.270979E-523.0012875
ATTAAAG1103.248076E-521.9600142
GTCATGA800.002889256721.5678711
CATAGAC800.002892497221.5637073
GTACTAC800.002892497221.5637073
CAATACT800.002892497221.5637074
ACTGCTA800.002895740821.5595448
Produced by FastQC (version 0.11.5)