FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr29.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr29.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189022
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAG350.001344964739.4285741
ATTAGCC400.002590872634.53
GCACCTT400.002590872634.56
TGAGCCG400.002590872634.55
GGCTAAA400.002590872634.51
CGTCATT851.1993689E-732.47058546
CAAGTCG751.6594859E-632.256
CGTCAAG751.6594859E-632.261
GCCATAG553.2468204E-431.3636341
GTGTACA553.2468204E-431.3636341
TTAGAGT450.004613025630.6666664
TAGTACT450.004613025630.6666664
AGTCGTC802.745579E-630.187558
GTCATTT1052.309207E-829.5714347
GTCAAGT953.179921E-729.05263162
TAGACAG605.405293E-428.755
AAGTCGT854.402482E-628.41176257
GGGTAGA500.007718845727.61
GTTAGTT500.007718845727.646
CTTATAG500.007718845727.61
Produced by FastQC (version 0.11.5)