FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr26.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr26.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160025
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6150.38431495078893924No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT150.00235191868.99937411
CGCCTCA1700.056.8230178
GTCTCCG1000.055.19953
TCTCCGC1250.055.19954
CGTCTCC850.052.7807242
CGACTCC200.007338709751.7657052
CGACCTT200.00734778851.749536
GACTCCG200.00734778851.749533
CCGTCTC900.049.8484571
CCGCCTC2000.048.2995647
TCCGCCT1650.048.0904736
CTCCGCC1950.045.9995845
CGCCACA451.8514183E-645.9995848
CCTCAGA2400.041.68712210
CTAGAAC602.8898648E-740.2496383
ACCGCCT602.8898648E-740.2496386
GTATAAG1550.040.0641520
GTCTAGA702.1631422E-839.440541
TCAGATG2050.038.70696612
GCCTCAG2500.038.639659
Produced by FastQC (version 0.11.5)