FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTGTGTTCTAAGTGGGAAGTGCACTCCCTCTGGACTGACGCTGTCTCTTATACACATCTGACGCTGCCGACT11.5384615384615385No Hit
ATTCATGAGATGGTTAGCTAGAACGGAACTCCCTAGTTAGAACCTGTCTCTTATACACATCTGACGCTGCCGACG11.5384615384615385No Hit
TTACATGCCAGTTGCCGTCCTCCTCTTCTCCTATCCCACCATCACAGCCCCTCCCCCAACTACCCCTTCCTCTTC11.5384615384615385No Hit
CTGTAAAGCACTCCTCTCTTGTCTCTAGAGTGGGTCTCTGCTTTTTGAGGACTGCCCTCTTGCTGTCTCTTATAC11.5384615384615385No Hit
GATATAATTGCACGCGAAAATTGCGGTTATCACCAAAAGCGCTGTCTCTTATACACATCTGACGCTGCCGACGAG11.5384615384615385No Hit
TCTATAATTATTGTCATCTTCATAAATTGTAAATATCAAACATTTTTGACCTTAATCATGTTTTTTGATAGATAT11.5384615384615385No Hit
TCGCAAGGGAGGCGGGGTGGGAGGCTCCGGAGATGCAGGCTTAGGGCCGCTGCGCTCTCTTGCACCCCTGCTCAC11.5384615384615385No Hit
GTCATATCCCGCCGGATATTCCAGGCATGGTGAAGCGCAAACTGGAAGCGAGCAATGATATCTACCAGACCTTTA11.5384615384615385No Hit
GCTATGGGTGCTGGGAGCAGCAAGGAAGCCTCCAACACAGGTCTGGGCCTTCTGCTCCAAGGTTGAGGGCGGGGC11.5384615384615385No Hit
GGTGGAAGCCGTGGCGTCTTCTCGGTCTTCTTAGCCAGACCCGGCCGGGCCAAGGAGGAAAGGCACCAGCCCCTC11.5384615384615385No Hit
ACCACAGGGAGTGGCACTATCAGGAGGTGTGGAGGAAGTGATTCAGTCTCCTCCGGTGAACTTAAGTTTGCAGGC11.5384615384615385No Hit
GTCTAGTTTTTGTCTGGGTAACCAGACTATTGCCAAGAGTAAAGTATTAAAATCTCCCACTATCAGTGTGAGAAG11.5384615384615385No Hit
GATACACACAGAGGTTTGTTTCCTAGATGTAACCCAAACCTATGTTCTTCCAACCTCTATTTATCCAGGTATTTA11.5384615384615385No Hit
CCTCTAAAAAGGCCAAGGGAAATGTTACATGCCTCCGTGGGAGATGAGAGGTGCGCAGAATGGGTTCTGTCTCTT11.5384615384615385No Hit
CCCTTAGACAGAAGCTACCAGTTCTCTCTGCCTTTCTGTTTGTTTCAGTTCTGGGACTCTAGGCATTGAGGGCCT11.5384615384615385No Hit
GGGTGAGGGTTAGGGTTCGGGGTAGGGTTAGGGTTCTGTCTCTTATACACATCTGACGCTGCCGACGATCGATCT11.5384615384615385No Hit
CAGAAGTCAACATCGAACAATGAAAATAAAAAACATCGTTGTAATACCACTAAATAAAAACCAAATTAGAAACAT11.5384615384615385No Hit
GCAGTATCACGTGCAACCGCGCCGGGTGCAGGATGGCGGCGGCGGCCGCGGCGGCAGCAGCGCTGGGTGTCAGGC11.5384615384615385No Hit
GACATATCCTATACAGAGATACTAAGAAAGGTGCCGAAAGAGAGAGAGATTCTAGGATGGCCTCTGAGCGGGAGC11.5384615384615385No Hit
TCCCTCCTCCCTTCAGTAGATTTAACACATCTCTTTCCCCCTCCTCCTTTACCTTCCCTGTCTCTTATACACATC11.5384615384615385No Hit
GCATGCGTGCGTGCGTGCGTGCGTGCGTGCGTGCGTGCGTGTGTGTGTGTGGTCTACCTGCTGCTTGTAACAGAT11.5384615384615385No Hit
GCCCAGCAGTCGCCGGGTCCTTGGCCCTACGTGCGACTCTTCAGAAGACACCGGCGAGTCTGGCTCTTATACACC11.5384615384615385No Hit
CATTCAGAGGAATACAGTCTGTATGAATGTGGAAAACATGTAGGCAGGTAGGGGAGGCACAGCAGCAAGAGCAGG11.5384615384615385No Hit
GCACTTACCATCAACTTTGTTGATCATCCTTCCGGACAAGTTAAATGTAAATTGGCTTTTGAGGACTCTTTGTTA11.5384615384615385No Hit
GCTGTGCTCCGCTCAAGTTACTGGACTTTGCACAGCTGGAACCAGCTGCTCTGGGCTGTAGCAGTTCTGTCTCTT11.5384615384615385No Hit
GATAAGGTGGGAGAGCCTGGGCGCGCGGCCGTGCGCGATCAGGGACGCGGCGGCCACCGGGGTCGAGGCCGCGGC11.5384615384615385No Hit
GGCTTCAGGAAGAAGTCAGTGTTCAGCATTTGAAACAGCTAAAACACCATTGTTGGGCCTGTCTCTTATACACAT11.5384615384615385No Hit
CTTAGAGCTTGAGCTAGCACTATGTTTATGGGCGGCCAGACAGAGATACCTCTTCTTTCGAATCTCCCAGTTCTC11.5384615384615385No Hit
TCCCCCACCTCCCCACACAAATCTTTCAAAACGTTTGGTTACCTATGCAGAGGTACCTCTGTCTCTTATACACAT11.5384615384615385No Hit
ACTCAAGCATGCACCCCTCTCTTAATTCAATGCCAGAAGTTCAATATTAACACACACACACACACAAACACACAC11.5384615384615385No Hit
CCCTTCCCTTTACTCCCTTCAAACCCTTCCATGTACCTCTCCCGATTCTCCTACAAATTCATGGCCTCTTTTCTC11.5384615384615385No Hit
GTCTAAGTCATCAGTGAGTGACCTCTTGACAAGCTGAAACCACAAAATGGGACTTATGGAGCCCGTGAAGCGGGC11.5384615384615385No Hit
CAATAACAAAGCCGAGAAATAACGGATAAATATAAAGTCTGATTATATTCATCATTATTGTCCTGATGATTCGGG11.5384615384615385No Hit
CTCCATGGGAGATGCATGGCATTCCATAAATCATAGATAGCCCGTGAGAAAACAAGACAAGAAAAGAACTAATCA11.5384615384615385No Hit
GCTCAGTTCCATGAGACCAAGTCACCATGGAGTTCAAACTGAAAGTGCTGTCTCTTATACACATCTGACGCTGCC11.5384615384615385No Hit
GGCCGCGCCTCCCTCCAGCTGTCACCACTCACCTGCTCTTCCGAGGCTGTCTCTTAGACACATCGGACGCTGCCG11.5384615384615385No Hit
ACATTGCTCTGTGACTGTCCTAGTGATGGAGGTAGGGTTAAGGGCTGTCTCTTATACACATCTGACGCTGCCGAC11.5384615384615385No Hit
ATCTCACCCTTGGCAGAGCACTGTCTGGAGATGTTGATCTCTGTCAAGTCGCAGAATGTCATGCCTGTCTCTTAT11.5384615384615385No Hit
CGTCTAAGAGGAGCGACGCCAAGCCTATGTTTCTCCAGCAGGTGGAGCTGTCTCTTATACACATCTACGCTGCCG11.5384615384615385No Hit
TCCAGGCCTTTCAGTTGTTTACTAGAAAGGACAGTCTTAAGCCAGATACAGTTTACCATAAGAAAAGTTAAAGAT11.5384615384615385No Hit
GCCCCACCTAGCAGGGGCGGGGAGCGACGTCAGCACGTCGGCCCTCACGTCCAGCTGGGTGACTACTGACCGGAA11.5384615384615385No Hit
CCCAGGGACCGCTTCCACATTACCACAGATGAATCGAGTCTGTGTTCCATGCCGAAAACCACAAACAAAAAAAAG11.5384615384615385No Hit
GCCCCGCCCCGCCCCCCACCTTGGGGCAGGTGAGCGGTGGCCAATGGGGGAGCGCGGGGCAGGTGCCCGCTAACT11.5384615384615385No Hit
GAATTCTGAGAATCAAATTTCTGTGGTGTAGTTGGACATTGGCTGAGATATTGCATTCTGTTGTAACTGTCTCTT11.5384615384615385No Hit
ATGATGCACAGCTGTGCTTTGATGAGGGGGAAGTGCAGAGTCTTCCTCCCTGCTGTGAGACCAGTTGGCCACCTG11.5384615384615385No Hit
CACGTATGTCACGAGCTATGAGAGTGTGGAACTTACTCACTATGATAGAATACAAGTTGAGGGGGGGGACATCGA11.5384615384615385No Hit
TGTTTGGGGTCTTTAACGAGCTGTCCCTTCGCTCTACTCCCGCAGGAAACGCTGTCTCTTATACACATCTGACGC11.5384615384615385No Hit
GCTGGTGAGACATCAGAAAAAGCTCAGAATCTATAGATAATCATCCTCAAAGTTCATCAGCAACAATATAAGACA11.5384615384615385No Hit
AAATTGCTCTTCTACTTCCACTACCATGAGCAATTCAAACAATTAAAACCTCTACTATAATAATTATAGCCTTTA11.5384615384615385No Hit
GTCTGAGGTTGATCGTTGCTAATTTGCAATTGAATAAAGTACCTGGACTTTACCATAGAAGCTTTTTTCTGTTGT11.5384615384615385No Hit
ATGCATGAGGCCTGCAGCCAATGGCCAGGCGAGGAGGTTGTTTTAATCGGCGGAGCCCGCTGGCCAATCAGGCGG11.5384615384615385No Hit
TTACACAAGAGGCGGCGACGCAAGTGCGCCTGCGCGGGATCTCCGTAGTCTGTGCTGAGGGCAGGAGCTGTCTCT11.5384615384615385No Hit
GCTGTCGCCTCTTTCACCGTTTTACAAACTTGTTGCCAGATCTCTGCCGAAGACTGTTCGACCTGTCTCTTATAC11.5384615384615385No Hit
TTCATATACCCCACAGGCCTGGACCACGGCCAGAACCCCTCACCAGAATGGAGAGAAAAATGTAGAGGTGATGGG11.5384615384615385No Hit
ACCCGTGACTCTAGAACTCGATAGGTAGGCCAGGAAGGCTACGAGCTCAGAGAACCTCCTACCACATTGCGATTT11.5384615384615385No Hit
GTGCTGATCTGTTCAGGAATTTTTCCACTGTGACAATATCTTCCAGGCTTTTCCGCACTTTCTCCTCAATCAGTG11.5384615384615385No Hit
GCATATAGCACCAGACCGATAGTCAGCGCCAGCCCTGCCAACACGGCACAGGCAATCCACAAGCGGTTTTTACGG11.5384615384615385No Hit
CTTGGTGGTCGGTTTGATTGTGCTGGTGCGTGGTCCTGGTGCTAAGGGCGGGGGGTGTCTGGTTTTGTGGGGGGT11.5384615384615385No Hit
TCTATGGACCTAATAGCATTGGTTATCCCATCTTACATTCTGCTTAAAGTACAGAGTATAAATCCAAGCACATCT11.5384615384615385No Hit
CGCTTATGGTCTGCAAGCGACGTCCCTGTAGCAAGGCAAACCATCGCTTCACGAGAGATGCTGAAAACGTAAGCT11.5384615384615385No Hit
ATAATGTTGAGAACACCACAGAAGATGGAGCTCTGGTTTGTGAAATTTCAGAGGGAAAATTAAAGACTCTTTTCA11.5384615384615385No Hit
GTGGCACACTTCCTCCAACAAGGCCACATCTCCTAATAGTAGCACTTCCATGGTCCAAGAAAATTCACACTACCA11.5384615384615385No Hit
CTACATGGCCGCACCGCTGACTCCTGCCTGGCCAGCAGGACCCTTCGAAACGCCGGTGCTCCACAGCTTACAGGG11.5384615384615385No Hit
GTGGGGGGGTGTTGATAAATAATTAACTTGGGTGGATTGCTTTGTGTCTGGAGCTGTCTCTTATACACATCTGAC11.5384615384615385No Hit
CGTTTATCAACCAGGGACAGGGAATCGCATAGAAATTAATGCCAGTCTCTTATAAACATCTGACTCTGCCGACGA11.5384615384615385No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAAACA50.069.061
ACAAAAA50.069.065
AAACCAC50.069.056
CACATTA50.069.016
AACAAAA50.069.064
GTGTTCC50.069.042
CCCAGGG50.069.01
ACAGATG50.069.025
TTACCAC50.069.020
AAACAAA50.069.063
GGACCGC50.069.06
TGCCGAA50.069.050
ACCACAG50.069.022
ACCACAA50.069.058
CCGCTTC50.069.09
GTTCCAT50.069.044
CACAAAC50.069.060
ACCGCTT50.069.08
CGCTTCC50.069.010
GAAAACC50.069.054