FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235350
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17490.7431485022307202No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTCGT400.002592981634.4977722
TCTCCGC807.057497E-834.497774
TAGTATC501.8576419E-434.497774
CTAGGGC751.6626182E-632.1979183
CTGAACG450.004616762530.6646849
GTATAAG756.136843E-527.6040820
GGATATG500.00772506927.5982176
CTAGTCT500.00772506927.5982174
GTCCTAC906.86772E-626.8373011
CGCCTCA658.639826E-426.5367458
ATAGTGT809.5343836E-525.8733278
TATAAGA1101.1447974E-625.0946221
TTTAAGG700.001331200824.6412643
CGAGCGA851.4371016E-424.3565469
TAAGACT902.1203083E-422.9985124
GCTAGGG953.0525553E-421.7926942
TAGCCTC953.056946E-421.7880655
AGCGCGG800.002893746421.5611065
TATACAG1304.8971597E-621.2293955
GGCTAGG1154.5774697E-521.0031031
Produced by FastQC (version 0.11.5)