FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33595
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTG252.527484E-455.2167175
TTTCCAC200.00728842951.76567516
CGACGAG658.4671495E-426.546569
CCGACGA900.005611610619.17247268
TGCCGAC1400.00401254214.79019267
ACGCTGC2050.003590872511.78405665
GCTGCCG2100.00429962811.50348267
CGCTGCC2150.005124983411.23595966
CACATCT4100.0081923877.575464267
TCTTATA5450.0017897576.96541169
CTCTTAT5650.00261798926.71884868
TGTCTCT6500.00159037866.371159665
TCTCTTA6000.0049156636.326915367
GTCTCTT6100.00583994766.223195666
CTGTCTC6750.00243298456.13519164
Produced by FastQC (version 0.11.5)