FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr143.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr143.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences501471
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7880.15713770088399925No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTACG451.8576247E-646.005982
GCCCGTA405.0000184E-543.122017
GACGATT350.001347551739.42583522
CTAAACG400.002593289634.504492
ACCCGTT400.002594558334.5010550
TTACCGT400.002594558334.5010534
ACGAATA400.002594558334.5010562
ACCTTCG400.002595827734.497617
CGGGTCG553.2538513E-431.36458852
TGCGCAA553.255755E-431.3614610
TAGGCCG553.255755E-431.361465
AACCCGT450.00461954630.66759949
CAAATCG450.00461954630.66759946
GACGAAT450.00461954630.66759961
CAATCGA450.00461954630.66759946
ATACCCG450.00461954630.66759943
GCGCATT703.845937E-529.57232738
CCGACGT854.417134E-628.4154669
GTATAGA1005.002512E-727.6035881
TGCCCGT500.007729684527.60083866