FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr142.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr142.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9542465
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG373810.39173316328642543No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATC77100.026.53631269
GAGCGAT120000.023.69115469
TATACCG3450.021.999465
GCGATCT50450.019.49054569
CGATCTA22200.019.2712169
CGAGCGA188750.018.09624769
ACGAGCG248100.017.95311269
TATACTG19550.017.4701585
CTACACT29350.016.573614
TAGACAG31650.015.47829155
GTATAGG13450.015.3914081
TACACCG10800.015.3329555
CTATACT19700.014.8853434
CTAGACT23350.014.7747194
GTGTAAG24550.014.616091
CGATCGA8500.014.61247869
CTAGGAC31200.014.5957183
GTAAGAC17350.014.5154173
GTCTAGA21600.014.3760021
TCTAGAC22100.014.3615623
Produced by FastQC (version 0.11.5)