FastQCFastQC Report
Mon 16 Mar 2020
HKVH7DRXX_n02_dpr141.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKVH7DRXX_n02_dpr141.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5216462
Sequences flagged as poor quality0
Sequence length75
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG145750.27940393316389533No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATAC6200.022.257963
AGCGATC31050.021.77976269
CGATCTA9150.021.49376169
GTATAGG6300.019.1680471
CTATACT8650.019.1444194
TATACTG6800.018.7719695
TAGACCG7300.018.431425
TAGACTG10100.017.0791265
GAGCGAT42850.016.90935968
GTTCTAC6150.016.830481
GCGATCT21550.016.0107469
CTAGACT9950.015.9496744
CTACACT15200.015.4341374
TACACTG10650.015.2252815
CTATACA7400.014.9188484
TAGACAG10950.014.8081495
GTACTAG4201.8189894E-1214.78677751
TGCCGAC195350.014.69496569
GTCTAAG8500.014.2069041
TTACACT9750.014.1537794
Produced by FastQC (version 0.11.5)